Peptide antibodies to the human c-fyn gene product demonstrate pp59c-fyn is capable of complex formation with the middle-T antigen of polyomavirus.

S. H. Cheng, R. Harvey, P. C. Espino, Kentaro Senba, T. Yamamoto, K. Toyoshima, A. E. Smith

研究成果: Article

69 引用 (Scopus)

抄録

The c-fyn proto-oncogene is a member of a family of closely related genes of which c-src is the prototype. Using peptide antibodies which had been raised against sequences predicted to be specific for the human c-fyn gene product, the c-fyn protein was identified. It is a tyrosine kinase with apparent mol. wt of 59 kd that is also phosphorylated and myristylated. Like pp60c-src and pp62c-yes, pp59c-fyn is able to form a stable complex with middle-T antigen, the transforming protein of polyomavirus. The transformation-defective middle-T mutant NG59, which is unable to associate stably with pp60c-src does not associate with pp59c-fyn. In contrast to pp60c-src, complex formation with middle-T antigen does not lead to a significant increase in the tyrosine kinase activity of pp59c-fyn. These findings lead us to suggest that middle-T mediated transformation may be a consequence of the deregulation of several members of the src-family of protein tyrosine kinases.

元の言語English
ページ(範囲)3845-3855
ページ数11
ジャーナルEMBO Journal
7
発行部数12
出版物ステータスPublished - 1988 12 1
外部発表Yes

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Polyomavirus Transforming Antigens
src-Family Kinases
Viral Tumor Antigens
Protein-Tyrosine Kinases
Proto-Oncogene Proteins c-fyn
Genes
src Genes
Polyomavirus
Peptides
Proto-Oncogenes
Antibodies
Deregulation
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

これを引用

Cheng, S. H., Harvey, R., Espino, P. C., Senba, K., Yamamoto, T., Toyoshima, K., & Smith, A. E. (1988). Peptide antibodies to the human c-fyn gene product demonstrate pp59c-fyn is capable of complex formation with the middle-T antigen of polyomavirus. EMBO Journal, 7(12), 3845-3855.

Peptide antibodies to the human c-fyn gene product demonstrate pp59c-fyn is capable of complex formation with the middle-T antigen of polyomavirus. / Cheng, S. H.; Harvey, R.; Espino, P. C.; Senba, Kentaro; Yamamoto, T.; Toyoshima, K.; Smith, A. E.

:: EMBO Journal, 巻 7, 番号 12, 01.12.1988, p. 3845-3855.

研究成果: Article

Cheng, SH, Harvey, R, Espino, PC, Senba, K, Yamamoto, T, Toyoshima, K & Smith, AE 1988, 'Peptide antibodies to the human c-fyn gene product demonstrate pp59c-fyn is capable of complex formation with the middle-T antigen of polyomavirus.', EMBO Journal, 巻. 7, 番号 12, pp. 3845-3855.
Cheng, S. H. ; Harvey, R. ; Espino, P. C. ; Senba, Kentaro ; Yamamoto, T. ; Toyoshima, K. ; Smith, A. E. / Peptide antibodies to the human c-fyn gene product demonstrate pp59c-fyn is capable of complex formation with the middle-T antigen of polyomavirus. :: EMBO Journal. 1988 ; 巻 7, 番号 12. pp. 3845-3855.
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