Peptide-based ligand screening system for g protein-coupled receptors using water-in-oil microdroplets

T. Sakurai; R. Iizuka; R. Sekine; Y. Nakamura; Donghyun Yoon; Tetsushi Sekiguchi; J. Ishii; A. Kondo; Shuichi Shoji; T. Funatsu

Peptide-based ligand screening system for g protein-coupled receptors using water-in-oil microdroplets

T. Sakurai, R. Iizuka^*, R. Sekine, Y. Nakamura, Donghyun Yoon, Tetsushi Sekiguchi, J. Ishii, A. Kondo, Shuichi Shoji, T. Funatsu

^*この研究の対応する著者

ナノ・ライフ創新研究機構

研究成果: Conference contribution

2 被引用数 (Scopus)

抄録

We present a novel peptide-based ligand screening system for G protein-coupled receptors (GPCRs) in water-in-oil (W/O) microdroplets. This system is an in vitro gene screening system for peptides that activate a target GPCR expressed in yeast. We show that the system is applicable to human GPCRs. This system will be a powerful tool for identifying novel peptide ligands for liganded mammalian GPCRs and orphan mammalian GPCRs.

本文言語	English
ホスト出版物のタイトル	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
出版社	Chemical and Biological Microsystems Society
ページ	965-967
ページ数	3
ISBN（印刷版）	9780979806476
出版ステータス	Published - 2014
イベント	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States 継続期間: 2014 10月 26 → 2014 10月 30

Other

Other	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
国/地域	United States
City	San Antonio
Period	14/10/26 → 14/10/30

ASJC Scopus subject areas

制御およびシステム工学

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引用スタイル

Sakurai, T., Iizuka, R., Sekine, R., Nakamura, Y., Yoon, D., Sekiguchi, T., Ishii, J., Kondo, A., Shoji, S., & Funatsu, T. (2014). Peptide-based ligand screening system for g protein-coupled receptors using water-in-oil microdroplets. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 965-967). Chemical and Biological Microsystems Society.

Sakurai, T, Iizuka, R, Sekine, R, Nakamura, Y, Yoon, D, Sekiguchi, T, Ishii, J, Kondo, A, Shoji, S & Funatsu, T 2014, Peptide-based ligand screening system for g protein-coupled receptors using water-in-oil microdroplets. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, pp. 965-967, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, San Antonio, United States, 14/10/26.

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title = "Peptide-based ligand screening system for g protein-coupled receptors using water-in-oil microdroplets",

abstract = "We present a novel peptide-based ligand screening system for G protein-coupled receptors (GPCRs) in water-in-oil (W/O) microdroplets. This system is an in vitro gene screening system for peptides that activate a target GPCR expressed in yeast. We show that the system is applicable to human GPCRs. This system will be a powerful tool for identifying novel peptide ligands for liganded mammalian GPCRs and orphan mammalian GPCRs.",

keywords = "Cell-free protein synthesis, G protein-coupled receptors, Ligand assay, Microdroplet, Yeast",

author = "T. Sakurai and R. Iizuka and R. Sekine and Y. Nakamura and Donghyun Yoon and Tetsushi Sekiguchi and J. Ishii and A. Kondo and Shuichi Shoji and T. Funatsu",

year = "2014",

language = "English",

isbn = "9780979806476",

pages = "965--967",

booktitle = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",

publisher = "Chemical and Biological Microsystems Society",

note = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 ; Conference date: 26-10-2014 Through 30-10-2014",

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TY - GEN

T1 - Peptide-based ligand screening system for g protein-coupled receptors using water-in-oil microdroplets

AU - Sakurai, T.

AU - Iizuka, R.

AU - Sekine, R.

AU - Nakamura, Y.

AU - Yoon, Donghyun

AU - Sekiguchi, Tetsushi

AU - Ishii, J.

AU - Kondo, A.

AU - Shoji, Shuichi

AU - Funatsu, T.

PY - 2014

Y1 - 2014

N2 - We present a novel peptide-based ligand screening system for G protein-coupled receptors (GPCRs) in water-in-oil (W/O) microdroplets. This system is an in vitro gene screening system for peptides that activate a target GPCR expressed in yeast. We show that the system is applicable to human GPCRs. This system will be a powerful tool for identifying novel peptide ligands for liganded mammalian GPCRs and orphan mammalian GPCRs.

AB - We present a novel peptide-based ligand screening system for G protein-coupled receptors (GPCRs) in water-in-oil (W/O) microdroplets. This system is an in vitro gene screening system for peptides that activate a target GPCR expressed in yeast. We show that the system is applicable to human GPCRs. This system will be a powerful tool for identifying novel peptide ligands for liganded mammalian GPCRs and orphan mammalian GPCRs.

KW - Cell-free protein synthesis

KW - G protein-coupled receptors

KW - Ligand assay

KW - Microdroplet

KW - Yeast

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M3 - Conference contribution

AN - SCOPUS:84941703173

SN - 9780979806476

SP - 965

EP - 967

BT - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

PB - Chemical and Biological Microsystems Society

T2 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Y2 - 26 October 2014 through 30 October 2014

ER -

Peptide-based ligand screening system for g protein-coupled receptors using water-in-oil microdroplets

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Other

ASJC Scopus subject areas

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