Persistent colonization of non-lymphoid tissue-resident macrophages by Stenotrophomonas maltophilia

Ichiro Takahashi, Koji Hosomi, Takahiro Nagatake, Hirokazu Toubou, Daiki Yamamoto, Ikue Hayashi, Yosuke Kurashima, Shintaro Sato, Naoko Shibata, Yoshiyuki Goto, Fumito Maruyama, Ichiro Nakagawa, Asaomi Kuwae, Akio Abe, Jun Kunisawa*, Hiroshi Kiyono

*この研究の対応する著者

研究成果: Article査読

2 被引用数 (Scopus)

抄録

Accumulating evidence has revealed that lymphoid tissue-resident commensal bacteria (e.g. Alcaligenes spp.) survive within dendritic cells. We extended our previous study by investigating microbes that persistently colonize colonic macrophages. 16S rRNA-based metagenome analysis using DNA purified from murine colonic macrophages revealed the presence of Stenotrophomonas maltophilia. The in situ intracellular colonization by S. maltophilia was recapitulated in vitro by using bone marrow-derived macrophages (BMDMs). Co-culture of BMDMs with clinically isolated S. maltophilia led to increased mitochondrial respiration and robust IL-10 production. We further identified a 25-kDa protein encoded by the gene assigned as smlt2713 (recently renamed as SMLT-RS12935) and secreted by S. maltophilia as the factor responsible for enhanced IL-10 production by BMDMs. IL-10 production is critical for maintenance of the symbiotic condition, because intracellular colonization by S. maltophilia was impaired in IL-10-deficient BMDMs, and smlt2713-deficient S. maltophilia failed to persistently colonize IL-10-competent BMDMs. These findings indicate a novel commensal network between colonic macrophages and S. maltophilia that is mediated by IL-10 and smlt2713.

本文言語English
ページ(範囲)133-141
ページ数9
ジャーナルInternational Immunology
32
2
DOI
出版ステータスPublished - 2019 11 12
外部発表はい

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学

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