Phase Separation of Polymerized Mixed Liposomes: Analysis of Release Behavior of Entrapped Molecules with Skeletonization

Shinji Takeoka, Hiromi Sakai, Hiroyuki Ohno, Eishun Tsuchida

研究成果: Article査読

11 被引用数 (Scopus)

抄録

The mixed liposomes, composed of a polymerizable lipid, l,2-bis(2,4-octadecadienoyl)-sn-glycero-3-phosphorylcholine (DODPC), and nonpolymerizable membrane constituents, DPPC, cholesterol, and sodium didodecyl phosphate, were prepared by an extrusion method. After polymerization, nonpolymerizable constituents were removed to obtain the polymerized framework of the liposome (the skeletonized liposome). The release of small molecules from the skeletonized liposomes through resulting holes was analyzed. A 5(6)-carboxyfluorescein (CF) and saccharides with various molecular weights were applied as release molecules. The molecular weight of dextran, whose retention ratio in the skeletonized liposomes is 50%, relates to the apparent size of the holes, i.e., the apparent domain size of the phase separation of a polymerized mixed liposome. The size of the holes increases with an increase in the mole fraction of nonpolymerizable lipids. This also depends on the polymerization temperature and the structure of nonpolymerizable lipids.

本文言語English
ページ(範囲)1279-1283
ページ数5
ジャーナルMacromolecules
24
6
DOI
出版ステータスPublished - 1991 11 1

ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics
  • Inorganic Chemistry
  • Materials Chemistry

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