Phenylalanine methyl ester production from phenylpyruvate methyl ester by immobilized Nocardia opaca under high hydrogen pressure

Noriyuki Nakamura, Anny Sulaswatty, Shigeo Nishimura, Tadashi Matsunaga, Takanori Kitamura, Masao Tsuji, Toshio Kawaguchi

研究成果: Article

4 引用 (Scopus)

抜粋

Phenylalanine methyl ester was produced by immobilized cells of Nocardia opaca from phenylpyruvate methyl ester and NH4Cl under high hydrogen pressure in a biphasic water-ethyl acetate mixture. The production rate (per dry cells) of phenylalanine methyl ester was 0.4 μmol g-1 min-1 under 100 bar hydrogen. When immobilized N. opaca were activated in the medium, the phenylalanine methyl ester production rate per gel increased from 12 to 20 nmol cm-3 min-1. Repeated batch production of phenylalanine methyl ester was carried out with periodic reactivation of immobilized cells. Immobilized cells retained about 70% of the initial activity after nine runs when they were activated in the medium and reacted in ethyl acetate-Tris-HCl buffer (80:20) system.

元の言語English
ページ(範囲)163-171
ページ数9
ジャーナルJournal of Biotechnology
26
発行部数2-3
DOI
出版物ステータスPublished - 1992
外部発表Yes

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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