Phenylalanine methyl ester was produced by immobilized cells of Nocardia opaca from phenylpyruvate methyl ester and NH4Cl under high hydrogen pressure in a biphasic water-ethyl acetate mixture. The production rate (per dry cells) of phenylalanine methyl ester was 0.4 μmol g-1 min-1 under 100 bar hydrogen. When immobilized N. opaca were activated in the medium, the phenylalanine methyl ester production rate per gel increased from 12 to 20 nmol cm-3 min-1. Repeated batch production of phenylalanine methyl ester was carried out with periodic reactivation of immobilized cells. Immobilized cells retained about 70% of the initial activity after nine runs when they were activated in the medium and reacted in ethyl acetate-Tris-HCl buffer (80:20) system.
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology