Phenylalanine methyl ester production from phenylpyruvate methyl ester by immobilized Nocardia opaca under high hydrogen pressure

Noriyuki Nakamura, Anny Sulaswatty, Shigeo Nishimura, Tadashi Matsunaga*, Takanori Kitamura, Masao Tsuji, Toshio Kawaguchi

*この研究の対応する著者

研究成果: Article査読

4 被引用数 (Scopus)

抄録

Phenylalanine methyl ester was produced by immobilized cells of Nocardia opaca from phenylpyruvate methyl ester and NH4Cl under high hydrogen pressure in a biphasic water-ethyl acetate mixture. The production rate (per dry cells) of phenylalanine methyl ester was 0.4 μmol g-1 min-1 under 100 bar hydrogen. When immobilized N. opaca were activated in the medium, the phenylalanine methyl ester production rate per gel increased from 12 to 20 nmol cm-3 min-1. Repeated batch production of phenylalanine methyl ester was carried out with periodic reactivation of immobilized cells. Immobilized cells retained about 70% of the initial activity after nine runs when they were activated in the medium and reacted in ethyl acetate-Tris-HCl buffer (80:20) system.

本文言語English
ページ(範囲)163-171
ページ数9
ジャーナルJournal of Biotechnology
26
2-3
DOI
出版ステータスPublished - 1992
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • 応用微生物学とバイオテクノロジー

フィンガープリント

「Phenylalanine methyl ester production from phenylpyruvate methyl ester by immobilized Nocardia opaca under high hydrogen pressure」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル