Phospholipase D is essential for keratocyte-like migration of NBT-II cells

Akira Nagasaki*, Kimiko Inotsume, Masamitsu Kanada, Taro Q.P. Uyeda

*この研究の対応する著者

研究成果: Article査読

9 被引用数 (Scopus)

抄録

NBT-II cells on collagen-coated substrates move rapidly and persistently, maintaining a semi-circular shape with a large lamellipodium, in a manner similar to fish keratocytes. The inhibitor of phospholipase D (PLD), n-butanol, completely blocked the migration and disturbed the characteristic localization of actin along the edge of lamellipodia. To investigate the functional difference between the two isozymes of PLD (PLD1 and PLD2), we transfected NBT-II cells with vectors expressing shRNA to deplete PLD1 or PLD2. Depletion of both PLD1 and 2 by RNA interference reduced the velocity of the migration, but depletion of PLD2 inhibited motility more severely than that of PLD1. Furthermore, GFP-PLD2 was localized to the protruding regions of lamellipodia in migrating cells. Thus, PLD is essential for the maintenance of keratocyte-like locomotion of NBT-II cells, presumably by regulating the actin cytoskeleton.

本文言語English
ページ(範囲)27-33
ページ数7
ジャーナルCell Structure and Function
33
1
DOI
出版ステータスPublished - 2008
外部発表はい

ASJC Scopus subject areas

  • 生理学
  • 分子生物学
  • 細胞生物学

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