Phosphorylated 5-ethynyl-2′-deoxyuridine for advanced DNA labeling

Siyoong Seo, Kazumitsu Onizuka, Chieko Nishioka, Eiki Takahashi, Satoshi Tsuneda, Hiroshi Abe*, Yoshihiro Ito

*この研究の対応する著者

研究成果: Article査読

11 被引用数 (Scopus)

抄録

The representative DNA-labeling agent 5-ethynyl-2′-deoxyuridine (EdU) was chemically modified to improve its function. Chemical monophosphorylation was expected to enhance the efficiency of the substrate in DNA polymerization by circumventing the enzymatic monophosphorylation step that consumes energy. In addition, to enhance cell permeability, the phosphates were protected with bis-pivaloyloxymethyl that is stable in buffer and plasma, and degradable inside various cell types. The phosphorylated EdU (PEdU) was less toxic than EdU, and had the same or a slightly higher DNA-labeling ability in vitro. PEdU was also successfully applied to DNA labeling in vivo. In conclusion, PEdU can be used as a less toxic DNA-labeling agent for studies that require long-term cell survival or very sensitive cell lines.

本文言語English
ページ(範囲)4589-4595
ページ数7
ジャーナルOrganic and Biomolecular Chemistry
13
15
DOI
出版ステータスPublished - 2015 4 21

ASJC Scopus subject areas

  • 生化学
  • 物理化学および理論化学
  • 有機化学

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