Photoactivation of a substrate for cell adhesion under standard fluorescence microscopes

Jun Nakanishi, Yukiko Kikuchi, Tohru Takarada, Hidekazu Nakayama, Kazuo Yamaguchi, Mizuo Maeda

研究成果: Article

149 引用 (Scopus)

抄録

Cell-culturing substrates where cell adhesion can be switched on by external stimuli during cell cultivation are useful scaffolds for tissue engineering, cell-based drug screening, and fundamental cellular studies. Here, we show a new strategy for photoactivation of a substrate for cell adhesion under standard fluorescence microscopes. A glass substrate chemically modified with an alkylsiloxane having a photocleavable 2-nitrobenzyl group was coated with bovine serum albumin to prevent cell adhesion. Upon irradiation under a fluorescence microscope, the protein was replaced with fibronectin, which made the irradiated region cell-adhesive. Subsequent seeding of HEK293 or COS7 cells produced patterns corresponding to the irradiated patterns. We succeeded for the first time in positioning single cells in proximity to cultivating single cells. The present method provides a general strategy for positioning single cells of same or different types at any locations on the substrate and will be useful for studying cell-cell interactions.

元の言語English
ページ(範囲)16314-16315
ページ数2
ジャーナルJournal of the American Chemical Society
126
発行部数50
DOI
出版物ステータスPublished - 2004 12 22
外部発表Yes

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Cell adhesion
Cell Adhesion
Microscopes
Fluorescence
Substrates
Scaffolds (biology)
Bovine Serum Albumin
Fibronectins
Tissue engineering
Adhesives
Screening
Irradiation
Proteins
Glass
Preclinical Drug Evaluations
Pharmaceutical Preparations
Tissue Engineering
Cell Communication

ASJC Scopus subject areas

  • Chemistry(all)

これを引用

Nakanishi, J., Kikuchi, Y., Takarada, T., Nakayama, H., Yamaguchi, K., & Maeda, M. (2004). Photoactivation of a substrate for cell adhesion under standard fluorescence microscopes. Journal of the American Chemical Society, 126(50), 16314-16315. https://doi.org/10.1021/ja044684c

Photoactivation of a substrate for cell adhesion under standard fluorescence microscopes. / Nakanishi, Jun; Kikuchi, Yukiko; Takarada, Tohru; Nakayama, Hidekazu; Yamaguchi, Kazuo; Maeda, Mizuo.

:: Journal of the American Chemical Society, 巻 126, 番号 50, 22.12.2004, p. 16314-16315.

研究成果: Article

Nakanishi, J, Kikuchi, Y, Takarada, T, Nakayama, H, Yamaguchi, K & Maeda, M 2004, 'Photoactivation of a substrate for cell adhesion under standard fluorescence microscopes', Journal of the American Chemical Society, 巻. 126, 番号 50, pp. 16314-16315. https://doi.org/10.1021/ja044684c
Nakanishi, Jun ; Kikuchi, Yukiko ; Takarada, Tohru ; Nakayama, Hidekazu ; Yamaguchi, Kazuo ; Maeda, Mizuo. / Photoactivation of a substrate for cell adhesion under standard fluorescence microscopes. :: Journal of the American Chemical Society. 2004 ; 巻 126, 番号 50. pp. 16314-16315.
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