Photochemical system for regenerating NADPH from NADP with use of immobilized chloroplasts

Isao Karube, Toshiaki Otsuka, Hiromichi Kayano, Tadashi Matsunaga, Shuichi Suzuki

研究成果: Article

15 引用 (Scopus)

抄録

Spinach chloroplasts were immobilized in 2% agar gel. Crude ferredoxin and NADP–ferredoxin oxidoreductase isolated from spinach were used as electron carriers. The activity of the NADP reduction by immobilized chloroplasts increased with increasing ferredoxin concentration and the maximum activity was obtained at 8μM ferredoxin. The saturation of NADP reduction was observed at a light intensity of over 1000 lx. The optimum pH and temperature of NADP reduction were 8 and 25°C, respectively. The reduced NADP in a reaction medium increased linearly with increasing reaction time under illumination. NADP was continuously reduced for 2 hr with a hollow‐fiber reactor containing immobilized chloroplasts. NADPH and NADP were separated with a hollow‐fiber dialyzer from ferredoxin and NADP–ferredoxin oxidoreductase, which were reused. The conversion ratio of NADP to NADPH was from 40 to 80%.

元の言語English
ページ(範囲)2655-2665
ページ数11
ジャーナルBiotechnology and Bioengineering
22
発行部数12
DOI
出版物ステータスPublished - 1980
外部発表Yes

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Chloroplasts
NADP
Ferredoxins
Spinacia oleracea
Gels
Lighting
Electrons
Oxidoreductases
Temperature
Agar
Light

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

これを引用

Photochemical system for regenerating NADPH from NADP with use of immobilized chloroplasts. / Karube, Isao; Otsuka, Toshiaki; Kayano, Hiromichi; Matsunaga, Tadashi; Suzuki, Shuichi.

:: Biotechnology and Bioengineering, 巻 22, 番号 12, 1980, p. 2655-2665.

研究成果: Article

Karube, Isao ; Otsuka, Toshiaki ; Kayano, Hiromichi ; Matsunaga, Tadashi ; Suzuki, Shuichi. / Photochemical system for regenerating NADPH from NADP with use of immobilized chloroplasts. :: Biotechnology and Bioengineering. 1980 ; 巻 22, 番号 12. pp. 2655-2665.
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abstract = "Spinach chloroplasts were immobilized in 2{\%} agar gel. Crude ferredoxin and NADP–ferredoxin oxidoreductase isolated from spinach were used as electron carriers. The activity of the NADP reduction by immobilized chloroplasts increased with increasing ferredoxin concentration and the maximum activity was obtained at 8μM ferredoxin. The saturation of NADP reduction was observed at a light intensity of over 1000 lx. The optimum pH and temperature of NADP reduction were 8 and 25°C, respectively. The reduced NADP in a reaction medium increased linearly with increasing reaction time under illumination. NADP was continuously reduced for 2 hr with a hollow‐fiber reactor containing immobilized chloroplasts. NADPH and NADP were separated with a hollow‐fiber dialyzer from ferredoxin and NADP–ferredoxin oxidoreductase, which were reused. The conversion ratio of NADP to NADPH was from 40 to 80{\%}.",
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AU - Otsuka, Toshiaki

AU - Kayano, Hiromichi

AU - Matsunaga, Tadashi

AU - Suzuki, Shuichi

PY - 1980

Y1 - 1980

N2 - Spinach chloroplasts were immobilized in 2% agar gel. Crude ferredoxin and NADP–ferredoxin oxidoreductase isolated from spinach were used as electron carriers. The activity of the NADP reduction by immobilized chloroplasts increased with increasing ferredoxin concentration and the maximum activity was obtained at 8μM ferredoxin. The saturation of NADP reduction was observed at a light intensity of over 1000 lx. The optimum pH and temperature of NADP reduction were 8 and 25°C, respectively. The reduced NADP in a reaction medium increased linearly with increasing reaction time under illumination. NADP was continuously reduced for 2 hr with a hollow‐fiber reactor containing immobilized chloroplasts. NADPH and NADP were separated with a hollow‐fiber dialyzer from ferredoxin and NADP–ferredoxin oxidoreductase, which were reused. The conversion ratio of NADP to NADPH was from 40 to 80%.

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