抄録
Spinach chloroplasts were immobilized in 2% agar gel. Crude ferredoxin and NADP–ferredoxin oxidoreductase isolated from spinach were used as electron carriers. The activity of the NADP reduction by immobilized chloroplasts increased with increasing ferredoxin concentration and the maximum activity was obtained at 8μM ferredoxin. The saturation of NADP reduction was observed at a light intensity of over 1000 lx. The optimum pH and temperature of NADP reduction were 8 and 25°C, respectively. The reduced NADP in a reaction medium increased linearly with increasing reaction time under illumination. NADP was continuously reduced for 2 hr with a hollow‐fiber reactor containing immobilized chloroplasts. NADPH and NADP were separated with a hollow‐fiber dialyzer from ferredoxin and NADP–ferredoxin oxidoreductase, which were reused. The conversion ratio of NADP to NADPH was from 40 to 80%.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 2655-2665 |
| ページ数 | 11 |
| ジャーナル | Biotechnology and Bioengineering |
| 巻 | 22 |
| 発行部数 | 12 |
| DOI | |
| 出版物ステータス | Published - 1980 |
| 外部発表 | Yes |
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ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
これを引用
Photochemical system for regenerating NADPH from NADP with use of immobilized chloroplasts. / Karube, Isao; Otsuka, Toshiaki; Kayano, Hiromichi; Matsunaga, Tadashi; Suzuki, Shuichi.
：: Biotechnology and Bioengineering, 巻 22, 番号 12, 1980, p. 2655-2665.研究成果: Article
}
TY - JOUR
T1 - Photochemical system for regenerating NADPH from NADP with use of immobilized chloroplasts
AU - Karube, Isao
AU - Otsuka, Toshiaki
AU - Kayano, Hiromichi
AU - Matsunaga, Tadashi
AU - Suzuki, Shuichi
PY - 1980
Y1 - 1980
N2 - Spinach chloroplasts were immobilized in 2% agar gel. Crude ferredoxin and NADP–ferredoxin oxidoreductase isolated from spinach were used as electron carriers. The activity of the NADP reduction by immobilized chloroplasts increased with increasing ferredoxin concentration and the maximum activity was obtained at 8μM ferredoxin. The saturation of NADP reduction was observed at a light intensity of over 1000 lx. The optimum pH and temperature of NADP reduction were 8 and 25°C, respectively. The reduced NADP in a reaction medium increased linearly with increasing reaction time under illumination. NADP was continuously reduced for 2 hr with a hollow‐fiber reactor containing immobilized chloroplasts. NADPH and NADP were separated with a hollow‐fiber dialyzer from ferredoxin and NADP–ferredoxin oxidoreductase, which were reused. The conversion ratio of NADP to NADPH was from 40 to 80%.
AB - Spinach chloroplasts were immobilized in 2% agar gel. Crude ferredoxin and NADP–ferredoxin oxidoreductase isolated from spinach were used as electron carriers. The activity of the NADP reduction by immobilized chloroplasts increased with increasing ferredoxin concentration and the maximum activity was obtained at 8μM ferredoxin. The saturation of NADP reduction was observed at a light intensity of over 1000 lx. The optimum pH and temperature of NADP reduction were 8 and 25°C, respectively. The reduced NADP in a reaction medium increased linearly with increasing reaction time under illumination. NADP was continuously reduced for 2 hr with a hollow‐fiber reactor containing immobilized chloroplasts. NADPH and NADP were separated with a hollow‐fiber dialyzer from ferredoxin and NADP–ferredoxin oxidoreductase, which were reused. The conversion ratio of NADP to NADPH was from 40 to 80%.
UR - http://www.scopus.com/inward/record.url?scp=84985715829&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84985715829&partnerID=8YFLogxK
U2 - 10.1002/bit.260221213
DO - 10.1002/bit.260221213
M3 - Article
AN - SCOPUS:84985715829
VL - 22
SP - 2655
EP - 2665
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
SN - 0006-3592
IS - 12
ER -