Photoconvertible pathogen labeling reveals nitric oxide control of leishmania major infection in vivo via dampening of parasite metabolism

Andreas J. Müller, Salome Aeschlimann, Romain Olekhnovitch, Mariko Dacher, Gerald F. Späth, Philippe Bousso

研究成果: Article

29 引用 (Scopus)

抄録

The immune system can control infectious diseases through different modes of action, including direct killing or spatial confinement. Addressing how the immune system impacts pathogen biology in vivo has remained challenging. We expressed a photoconvertible fluorescent protein in pathogens in order to track their spatial dissemination in infected tissues. In addition, we developed the fluorescence recovery after photoconversion (FRAC) method in order to probe pathogen metabolic activity in vivo. Combining these two approaches in the context of Leishmania major infection of mice and pharmacologically inhibiting iNOS, we found that nitric oxide produced during the immune response to L. major reduces the metabolic activity of intracellular parasites without necessarily exerting direct killing. We propose that this chronic pressure on pathogen proliferation represents a sublethal mode of control required for ultimately resolving the infection. The ability to probe pathogen biology in response to immune defense mechanisms in vivo should create opportunities for better dissecting host-pathogen interactions.

元の言語English
ページ(範囲)460-467
ページ数8
ジャーナルCell Host and Microbe
14
発行部数4
DOI
出版物ステータスPublished - 2013 10 16
外部発表Yes

Fingerprint

Leishmania major
Immune System
Nitric Oxide
Parasites
Host-Pathogen Interactions
Infection
Communicable Diseases
Fluorescence
Pressure
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology and Microbiology(all)
  • Cancer Research

これを引用

Photoconvertible pathogen labeling reveals nitric oxide control of leishmania major infection in vivo via dampening of parasite metabolism. / Müller, Andreas J.; Aeschlimann, Salome; Olekhnovitch, Romain; Dacher, Mariko; Späth, Gerald F.; Bousso, Philippe.

:: Cell Host and Microbe, 巻 14, 番号 4, 16.10.2013, p. 460-467.

研究成果: Article

Müller, Andreas J. ; Aeschlimann, Salome ; Olekhnovitch, Romain ; Dacher, Mariko ; Späth, Gerald F. ; Bousso, Philippe. / Photoconvertible pathogen labeling reveals nitric oxide control of leishmania major infection in vivo via dampening of parasite metabolism. :: Cell Host and Microbe. 2013 ; 巻 14, 番号 4. pp. 460-467.
@article{dcf9ed12c7c64da4936173c9136e7ac7,
title = "Photoconvertible pathogen labeling reveals nitric oxide control of leishmania major infection in vivo via dampening of parasite metabolism",
abstract = "The immune system can control infectious diseases through different modes of action, including direct killing or spatial confinement. Addressing how the immune system impacts pathogen biology in vivo has remained challenging. We expressed a photoconvertible fluorescent protein in pathogens in order to track their spatial dissemination in infected tissues. In addition, we developed the fluorescence recovery after photoconversion (FRAC) method in order to probe pathogen metabolic activity in vivo. Combining these two approaches in the context of Leishmania major infection of mice and pharmacologically inhibiting iNOS, we found that nitric oxide produced during the immune response to L. major reduces the metabolic activity of intracellular parasites without necessarily exerting direct killing. We propose that this chronic pressure on pathogen proliferation represents a sublethal mode of control required for ultimately resolving the infection. The ability to probe pathogen biology in response to immune defense mechanisms in vivo should create opportunities for better dissecting host-pathogen interactions.",
author = "M{\"u}ller, {Andreas J.} and Salome Aeschlimann and Romain Olekhnovitch and Mariko Dacher and Sp{\"a}th, {Gerald F.} and Philippe Bousso",
year = "2013",
month = "10",
day = "16",
doi = "10.1016/j.chom.2013.09.008",
language = "English",
volume = "14",
pages = "460--467",
journal = "Cell Host and Microbe",
issn = "1931-3128",
publisher = "Cell Press",
number = "4",

}

TY - JOUR

T1 - Photoconvertible pathogen labeling reveals nitric oxide control of leishmania major infection in vivo via dampening of parasite metabolism

AU - Müller, Andreas J.

AU - Aeschlimann, Salome

AU - Olekhnovitch, Romain

AU - Dacher, Mariko

AU - Späth, Gerald F.

AU - Bousso, Philippe

PY - 2013/10/16

Y1 - 2013/10/16

N2 - The immune system can control infectious diseases through different modes of action, including direct killing or spatial confinement. Addressing how the immune system impacts pathogen biology in vivo has remained challenging. We expressed a photoconvertible fluorescent protein in pathogens in order to track their spatial dissemination in infected tissues. In addition, we developed the fluorescence recovery after photoconversion (FRAC) method in order to probe pathogen metabolic activity in vivo. Combining these two approaches in the context of Leishmania major infection of mice and pharmacologically inhibiting iNOS, we found that nitric oxide produced during the immune response to L. major reduces the metabolic activity of intracellular parasites without necessarily exerting direct killing. We propose that this chronic pressure on pathogen proliferation represents a sublethal mode of control required for ultimately resolving the infection. The ability to probe pathogen biology in response to immune defense mechanisms in vivo should create opportunities for better dissecting host-pathogen interactions.

AB - The immune system can control infectious diseases through different modes of action, including direct killing or spatial confinement. Addressing how the immune system impacts pathogen biology in vivo has remained challenging. We expressed a photoconvertible fluorescent protein in pathogens in order to track their spatial dissemination in infected tissues. In addition, we developed the fluorescence recovery after photoconversion (FRAC) method in order to probe pathogen metabolic activity in vivo. Combining these two approaches in the context of Leishmania major infection of mice and pharmacologically inhibiting iNOS, we found that nitric oxide produced during the immune response to L. major reduces the metabolic activity of intracellular parasites without necessarily exerting direct killing. We propose that this chronic pressure on pathogen proliferation represents a sublethal mode of control required for ultimately resolving the infection. The ability to probe pathogen biology in response to immune defense mechanisms in vivo should create opportunities for better dissecting host-pathogen interactions.

UR - http://www.scopus.com/inward/record.url?scp=84886047191&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84886047191&partnerID=8YFLogxK

U2 - 10.1016/j.chom.2013.09.008

DO - 10.1016/j.chom.2013.09.008

M3 - Article

C2 - 24139402

AN - SCOPUS:84886047191

VL - 14

SP - 460

EP - 467

JO - Cell Host and Microbe

JF - Cell Host and Microbe

SN - 1931-3128

IS - 4

ER -