Photoconvertible pathogen labeling reveals nitric oxide control of leishmania major infection in vivo via dampening of parasite metabolism

Andreas J. Müller*, Salome Aeschlimann, Romain Olekhnovitch, Mariko Dacher, Gerald F. Späth, Philippe Bousso

*この研究の対応する著者

研究成果: Article査読

48 被引用数 (Scopus)

抄録

The immune system can control infectious diseases through different modes of action, including direct killing or spatial confinement. Addressing how the immune system impacts pathogen biology in vivo has remained challenging. We expressed a photoconvertible fluorescent protein in pathogens in order to track their spatial dissemination in infected tissues. In addition, we developed the fluorescence recovery after photoconversion (FRAC) method in order to probe pathogen metabolic activity in vivo. Combining these two approaches in the context of Leishmania major infection of mice and pharmacologically inhibiting iNOS, we found that nitric oxide produced during the immune response to L. major reduces the metabolic activity of intracellular parasites without necessarily exerting direct killing. We propose that this chronic pressure on pathogen proliferation represents a sublethal mode of control required for ultimately resolving the infection. The ability to probe pathogen biology in response to immune defense mechanisms in vivo should create opportunities for better dissecting host-pathogen interactions.

本文言語English
ページ(範囲)460-467
ページ数8
ジャーナルCell Host and Microbe
14
4
DOI
出版ステータスPublished - 2013 10月 16
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 免疫学および微生物学(全般)
  • 癌研究

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