抄録
The immune system can control infectious diseases through different modes of action, including direct killing or spatial confinement. Addressing how the immune system impacts pathogen biology in vivo has remained challenging. We expressed a photoconvertible fluorescent protein in pathogens in order to track their spatial dissemination in infected tissues. In addition, we developed the fluorescence recovery after photoconversion (FRAC) method in order to probe pathogen metabolic activity in vivo. Combining these two approaches in the context of Leishmania major infection of mice and pharmacologically inhibiting iNOS, we found that nitric oxide produced during the immune response to L. major reduces the metabolic activity of intracellular parasites without necessarily exerting direct killing. We propose that this chronic pressure on pathogen proliferation represents a sublethal mode of control required for ultimately resolving the infection. The ability to probe pathogen biology in response to immune defense mechanisms in vivo should create opportunities for better dissecting host-pathogen interactions.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 460-467 |
| ページ数 | 8 |
| ジャーナル | Cell Host and Microbe |
| 巻 | 14 |
| 発行部数 | 4 |
| DOI | |
| 出版物ステータス | Published - 2013 10 16 |
| 外部発表 | Yes |
Fingerprint
ASJC Scopus subject areas
- Molecular Biology
- Immunology and Microbiology(all)
- Cancer Research
これを引用
Photoconvertible pathogen labeling reveals nitric oxide control of leishmania major infection in vivo via dampening of parasite metabolism. / Müller, Andreas J.; Aeschlimann, Salome; Olekhnovitch, Romain; Dacher, Mariko; Späth, Gerald F.; Bousso, Philippe.
：: Cell Host and Microbe, 巻 14, 番号 4, 16.10.2013, p. 460-467.研究成果: Article
}
TY - JOUR
T1 - Photoconvertible pathogen labeling reveals nitric oxide control of leishmania major infection in vivo via dampening of parasite metabolism
AU - Müller, Andreas J.
AU - Aeschlimann, Salome
AU - Olekhnovitch, Romain
AU - Dacher, Mariko
AU - Späth, Gerald F.
AU - Bousso, Philippe
PY - 2013/10/16
Y1 - 2013/10/16
N2 - The immune system can control infectious diseases through different modes of action, including direct killing or spatial confinement. Addressing how the immune system impacts pathogen biology in vivo has remained challenging. We expressed a photoconvertible fluorescent protein in pathogens in order to track their spatial dissemination in infected tissues. In addition, we developed the fluorescence recovery after photoconversion (FRAC) method in order to probe pathogen metabolic activity in vivo. Combining these two approaches in the context of Leishmania major infection of mice and pharmacologically inhibiting iNOS, we found that nitric oxide produced during the immune response to L. major reduces the metabolic activity of intracellular parasites without necessarily exerting direct killing. We propose that this chronic pressure on pathogen proliferation represents a sublethal mode of control required for ultimately resolving the infection. The ability to probe pathogen biology in response to immune defense mechanisms in vivo should create opportunities for better dissecting host-pathogen interactions.
AB - The immune system can control infectious diseases through different modes of action, including direct killing or spatial confinement. Addressing how the immune system impacts pathogen biology in vivo has remained challenging. We expressed a photoconvertible fluorescent protein in pathogens in order to track their spatial dissemination in infected tissues. In addition, we developed the fluorescence recovery after photoconversion (FRAC) method in order to probe pathogen metabolic activity in vivo. Combining these two approaches in the context of Leishmania major infection of mice and pharmacologically inhibiting iNOS, we found that nitric oxide produced during the immune response to L. major reduces the metabolic activity of intracellular parasites without necessarily exerting direct killing. We propose that this chronic pressure on pathogen proliferation represents a sublethal mode of control required for ultimately resolving the infection. The ability to probe pathogen biology in response to immune defense mechanisms in vivo should create opportunities for better dissecting host-pathogen interactions.
UR - http://www.scopus.com/inward/record.url?scp=84886047191&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84886047191&partnerID=8YFLogxK
U2 - 10.1016/j.chom.2013.09.008
DO - 10.1016/j.chom.2013.09.008
M3 - Article
C2 - 24139402
AN - SCOPUS:84886047191
VL - 14
SP - 460
EP - 467
JO - Cell Host and Microbe
JF - Cell Host and Microbe
SN - 1931-3128
IS - 4
ER -