We synthesized poly(N-isopropylacrylamide) terminated with psoralen. Photo-induced reaction between DNA and the psoralen end group resulted in the grafting of poly(N-isopropylacrylamide) on double-stranded DNA. On the other hand, binding efficiency of DNA binding proteins such as restriction endonucleases to the DNA decreased significantly with increasing degree of polymer modification: At a certain level of polymer introduction, DNA was revealed to be practically 'jacketed'. Lithography was attempted to this grafting procedure. We found that pre-incubation of a restriction endonuclease EcoRI with DNA resulted in selective preservation of its binding site from polymer modification. The application of this technique would allow us facile methods to construct a precisely regulated nanoscale architecture.
|出版ステータス||Published - 1998|
|イベント||Proceedings of the 11th Toyota Conference on Nanostructured Materials in Biological and Artificial Systems - Shizuoka, Jpn|
継続期間: 1997 11月 5 → 1997 11月 8
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