PHOTOSENSITIVITIES OF IODOPSIN AND RHODOPSINS

Toshiyuki Okano, Yoshitaka Fukada, Yoshinori Shichida, Tôru Yoshizawa*

*この研究の対応する著者

研究成果: Article査読

48 被引用数 (Scopus)

抄録

Abstract— The relative photosensitivity and the molar extinction coefficient of a highly purified iodopsin (chicken red sensitive cone visual pigment) solubilized in a mixture of 3–[(3‐cholamidopropyI)‐dimethylammonio]‐1‐propanesulfonate and phosphatidylcholine (CHAPS‐PC) were measured using bovine rhodopsin solubilized in 2% digitonin as a standard and compared with those of chicken and bovine rhodopsins. The photosensitivity obtained (1.08) was close to those of rhodopsins (chicken, 1.04; bovine, 0.99) in CHAPS‐PC. The molar extinction coefficient of iodopsin (47200) was 1.15–1.17 times higher than those of rhodopsins (chicken, 40500; bovine, 41200). The oscillator strength of iodopsin (0.60) calculated from the extinction coefficient was nearly identical to that of chicken rhodopsin (0.61), suggesting that the chromophore of iodopsin is similar in configuration to rhodopsin. In contrast, the difference in quantum yield between iodopsin (0.62) and chicken rhodopsin (0.70) suggests that the chromophore‐opsin interaction after absorption of a photon by the chromophore may be different.

本文言語English
ページ(範囲)995-1001
ページ数7
ジャーナルPhotochemistry and Photobiology
56
6
DOI
出版ステータスPublished - 1992 12月
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 物理化学および理論化学

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