Physical interactions among circadian clock proteins KaiA, KaiB and KaiC in cyanobacteria

Hideo Iwasaki, Yasuhito Taniguchi, Masahiro Ishiura, Takao Kondo

研究成果: Article

129 引用 (Scopus)

抄録

The kai gene cluster, which is composed of three genes, kaiA, kaiB and kaiC, is essential for the generation of circadian rhythms in the unicellular cyanobacterium Synechococcus sp. strain PCC 7942. Here we demonstrate the direct association of KaiA, KaiB and KaiC in yeast cells using the two-hybrid system, in vitro and in cyanobacterial cells. KaiC enhanced KaiA-KaiB interaction in vitro and in yeast cells, suggesting that the three Kai proteins were able to form a heteromultimeric complex. We also found that a long period mutation kaiA1 dramatically enhanced KaiA-KaiB interaction in vitro. Thus, direct protein-protein association among the Kai proteins may be a critical process in the generation of circadian rhythms in cyanobacteria.

元の言語English
ページ(範囲)1137-1145
ページ数9
ジャーナルEMBO Journal
18
発行部数5
出版物ステータスPublished - 1999 3 1
外部発表Yes

Fingerprint

Circadian Clocks
Cyanobacteria
Clocks
Circadian Rhythm
Yeast
Proteins
Genes
Yeasts
Cells
Synechococcus
Multigene Family
Hybrid systems
Mutation
In Vitro Techniques

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

これを引用

Physical interactions among circadian clock proteins KaiA, KaiB and KaiC in cyanobacteria. / Iwasaki, Hideo; Taniguchi, Yasuhito; Ishiura, Masahiro; Kondo, Takao.

:: EMBO Journal, 巻 18, 番号 5, 01.03.1999, p. 1137-1145.

研究成果: Article

Iwasaki, H, Taniguchi, Y, Ishiura, M & Kondo, T 1999, 'Physical interactions among circadian clock proteins KaiA, KaiB and KaiC in cyanobacteria', EMBO Journal, 巻. 18, 番号 5, pp. 1137-1145.
Iwasaki, Hideo ; Taniguchi, Yasuhito ; Ishiura, Masahiro ; Kondo, Takao. / Physical interactions among circadian clock proteins KaiA, KaiB and KaiC in cyanobacteria. :: EMBO Journal. 1999 ; 巻 18, 番号 5. pp. 1137-1145.
@article{91ffdde3dc314d52a50e215aa8b49727,
title = "Physical interactions among circadian clock proteins KaiA, KaiB and KaiC in cyanobacteria",
abstract = "The kai gene cluster, which is composed of three genes, kaiA, kaiB and kaiC, is essential for the generation of circadian rhythms in the unicellular cyanobacterium Synechococcus sp. strain PCC 7942. Here we demonstrate the direct association of KaiA, KaiB and KaiC in yeast cells using the two-hybrid system, in vitro and in cyanobacterial cells. KaiC enhanced KaiA-KaiB interaction in vitro and in yeast cells, suggesting that the three Kai proteins were able to form a heteromultimeric complex. We also found that a long period mutation kaiA1 dramatically enhanced KaiA-KaiB interaction in vitro. Thus, direct protein-protein association among the Kai proteins may be a critical process in the generation of circadian rhythms in cyanobacteria.",
keywords = "Circadian rhythm, Kai genes, Protein-protein interaction, Synechococcus",
author = "Hideo Iwasaki and Yasuhito Taniguchi and Masahiro Ishiura and Takao Kondo",
year = "1999",
month = "3",
day = "1",
language = "English",
volume = "18",
pages = "1137--1145",
journal = "EMBO Journal",
issn = "0261-4189",
publisher = "Nature Publishing Group",
number = "5",

}

TY - JOUR

T1 - Physical interactions among circadian clock proteins KaiA, KaiB and KaiC in cyanobacteria

AU - Iwasaki, Hideo

AU - Taniguchi, Yasuhito

AU - Ishiura, Masahiro

AU - Kondo, Takao

PY - 1999/3/1

Y1 - 1999/3/1

N2 - The kai gene cluster, which is composed of three genes, kaiA, kaiB and kaiC, is essential for the generation of circadian rhythms in the unicellular cyanobacterium Synechococcus sp. strain PCC 7942. Here we demonstrate the direct association of KaiA, KaiB and KaiC in yeast cells using the two-hybrid system, in vitro and in cyanobacterial cells. KaiC enhanced KaiA-KaiB interaction in vitro and in yeast cells, suggesting that the three Kai proteins were able to form a heteromultimeric complex. We also found that a long period mutation kaiA1 dramatically enhanced KaiA-KaiB interaction in vitro. Thus, direct protein-protein association among the Kai proteins may be a critical process in the generation of circadian rhythms in cyanobacteria.

AB - The kai gene cluster, which is composed of three genes, kaiA, kaiB and kaiC, is essential for the generation of circadian rhythms in the unicellular cyanobacterium Synechococcus sp. strain PCC 7942. Here we demonstrate the direct association of KaiA, KaiB and KaiC in yeast cells using the two-hybrid system, in vitro and in cyanobacterial cells. KaiC enhanced KaiA-KaiB interaction in vitro and in yeast cells, suggesting that the three Kai proteins were able to form a heteromultimeric complex. We also found that a long period mutation kaiA1 dramatically enhanced KaiA-KaiB interaction in vitro. Thus, direct protein-protein association among the Kai proteins may be a critical process in the generation of circadian rhythms in cyanobacteria.

KW - Circadian rhythm

KW - Kai genes

KW - Protein-protein interaction

KW - Synechococcus

UR - http://www.scopus.com/inward/record.url?scp=0033104504&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033104504&partnerID=8YFLogxK

M3 - Article

C2 - 10064581

AN - SCOPUS:0033104504

VL - 18

SP - 1137

EP - 1145

JO - EMBO Journal

JF - EMBO Journal

SN - 0261-4189

IS - 5

ER -