Physical interactions among circadian clock proteins KaiA, KaiB and KaiC in cyanobacteria

Hideo Iwasaki, Yasuhito Taniguchi, Masahiro Ishiura, Takao Kondo

研究成果: Article

131 引用 (Scopus)

抜粋

The kai gene cluster, which is composed of three genes, kaiA, kaiB and kaiC, is essential for the generation of circadian rhythms in the unicellular cyanobacterium Synechococcus sp. strain PCC 7942. Here we demonstrate the direct association of KaiA, KaiB and KaiC in yeast cells using the two-hybrid system, in vitro and in cyanobacterial cells. KaiC enhanced KaiA-KaiB interaction in vitro and in yeast cells, suggesting that the three Kai proteins were able to form a heteromultimeric complex. We also found that a long period mutation kaiA1 dramatically enhanced KaiA-KaiB interaction in vitro. Thus, direct protein-protein association among the Kai proteins may be a critical process in the generation of circadian rhythms in cyanobacteria.

元の言語English
ページ(範囲)1137-1145
ページ数9
ジャーナルEMBO Journal
18
発行部数5
出版物ステータスPublished - 1999 3 1
外部発表Yes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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