Physical interactions among circadian clock proteins KaiA, KaiB and KaiC in cyanobacteria

Hideo Iwasaki, Yasuhito Taniguchi, Masahiro Ishiura, Takao Kondo*

*この研究の対応する著者

研究成果: Article査読

135 被引用数 (Scopus)

抄録

The kai gene cluster, which is composed of three genes, kaiA, kaiB and kaiC, is essential for the generation of circadian rhythms in the unicellular cyanobacterium Synechococcus sp. strain PCC 7942. Here we demonstrate the direct association of KaiA, KaiB and KaiC in yeast cells using the two-hybrid system, in vitro and in cyanobacterial cells. KaiC enhanced KaiA-KaiB interaction in vitro and in yeast cells, suggesting that the three Kai proteins were able to form a heteromultimeric complex. We also found that a long period mutation kaiA1 dramatically enhanced KaiA-KaiB interaction in vitro. Thus, direct protein-protein association among the Kai proteins may be a critical process in the generation of circadian rhythms in cyanobacteria.

本文言語English
ページ(範囲)1137-1145
ページ数9
ジャーナルEMBO Journal
18
5
DOI
出版ステータスPublished - 1999 3 1
外部発表はい

ASJC Scopus subject areas

  • 神経科学(全般)
  • 分子生物学
  • 生化学、遺伝学、分子生物学(全般)
  • 免疫学および微生物学(全般)

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