Platelet-derived growth factor oncoprotein detection using three-dimensional carbon microarrays

Varun Penmatsa, A. Rahim Ruslinda, Majid Beidaghi, Hiroshi Kawarada, Chunlei Wang*

*この研究の対応する著者

研究成果: Article査読

31 被引用数 (Scopus)

抄録

The potential of aptamers as ligand binding molecule has opened new avenues in the development of biosensors for cancer oncoproteins. In this paper, a label-free detection strategy using signaling aptamer/protein binding complex for platelet-derived growth factor (PDGF-BB) oncoprotein detection is reported. The detection mechanism is based on the release of fluorophore (TOTO intercalating dye) from the target binding aptamer's stem structure when it captures PDGF. Amino-terminated three-dimensional carbon microarrays fabricated by pyrolyzing patterned photoresist were used as a detection platform. The sensor showed near linear relationship between the relative fluorescence difference and protein concentration even in the sub-nanomolar range with an excellent detection limit of 5. pmol. This detection strategy is promising in a wide range of applications in the detection of cancer biomarkers and other proteins.

本文言語English
ページ(範囲)118-123
ページ数6
ジャーナルBiosensors and Bioelectronics
39
1
DOI
出版ステータスPublished - 2013 1月 15

ASJC Scopus subject areas

  • バイオテクノロジー
  • 生物理学
  • 生体医工学
  • 電気化学

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