@article{3bfa36c9e8384149b58d73358708af9c,
title = "Platelet-derived growth factor oncoprotein detection using three-dimensional carbon microarrays",
abstract = "The potential of aptamers as ligand binding molecule has opened new avenues in the development of biosensors for cancer oncoproteins. In this paper, a label-free detection strategy using signaling aptamer/protein binding complex for platelet-derived growth factor (PDGF-BB) oncoprotein detection is reported. The detection mechanism is based on the release of fluorophore (TOTO intercalating dye) from the target binding aptamer's stem structure when it captures PDGF. Amino-terminated three-dimensional carbon microarrays fabricated by pyrolyzing patterned photoresist were used as a detection platform. The sensor showed near linear relationship between the relative fluorescence difference and protein concentration even in the sub-nanomolar range with an excellent detection limit of 5. pmol. This detection strategy is promising in a wide range of applications in the detection of cancer biomarkers and other proteins.",
keywords = "3D carbon microarrays, Direct amination, Fluorescence detection, Platelet-derived growth factor",
author = "Varun Penmatsa and Ruslinda, {A. Rahim} and Majid Beidaghi and Hiroshi Kawarada and Chunlei Wang",
note = "Funding Information: Varun Penmatsa and Majid Beidaghi gratefully acknowledges the financial support from the DEA and DYF fellowships provided by FIU graduate school . This project is partially supported by the National Science Foundation ( OISE 0934078 and CMMI 0800525 ). The authors would also like to acknowledge Grant-in-Aid from GCOE Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan and a Grant-in-Aid for Fundamental Research of Japan Society for the Promotion of Science (JSPS) ( 23246069 ). The authors would like to acknowledge the research facilities provided at AMERI at FIU and NTRC at Waseda University.",
year = "2013",
month = jan,
day = "15",
doi = "10.1016/j.bios.2012.06.055",
language = "English",
volume = "39",
pages = "118--123",
journal = "Biosensors and Bioelectronics",
issn = "0956-5663",
publisher = "Elsevier Ltd",
number = "1",
}