PLC β4-independent Ca2+ rise via muscarinic receptors in the mouse suprachiasmatic nucleus

Masayuki Ikeda, Takashi Sugiyama, Kaoru Suzuki, Takahiro Moriya, Shigenobu Shibata, Motoya Katsuki, Charles N. Allen, Tohru Yoshioka

    研究成果: Article

    13 引用 (Scopus)

    抄録

    Cholinergic regulation of the suprachiasmatic nucleus (SCN) has been extensively studied although the intracellular signaling mechanisms are not well understood. We examined immunostaining for phospholipase C-β (PLC-β) families that couple to muscarinic acetylcholine receptors (mAChR) and demonstrated the expression of PLC-βI and β4 in the mouse SCN. Ca2+ imaging analysis indicated that the MI-mAChR antagonist, pirenzepine blocked carbachol-induced Ca2+ elevation in the SCN and the response was equivalent between the wild type and the PLC-β4-knockout mice. In addition, the knockout mice displayed locomotor and temperature rhythms coupling to 24 h light/dark cycles. Therefore, it was proposed that PLC-βI but not PLC-β4 was involved in the mAChR-mediated Ca2+ signaling in the SCN. (C) 2000 Lippincott Williams and Wilkins.

    元の言語English
    ページ(範囲)907-912
    ページ数6
    ジャーナルNeuroReport
    11
    発行部数5
    出版物ステータスPublished - 2000 4 7

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    Suprachiasmatic Nucleus
    Type C Phospholipases
    Muscarinic Receptors
    Knockout Mice
    Pirenzepine
    Photoperiod
    Cholinergic Antagonists
    Carbachol
    Cholinergic Agents
    Temperature

    ASJC Scopus subject areas

    • Neuroscience(all)

    これを引用

    Ikeda, M., Sugiyama, T., Suzuki, K., Moriya, T., Shibata, S., Katsuki, M., ... Yoshioka, T. (2000). PLC β4-independent Ca2+ rise via muscarinic receptors in the mouse suprachiasmatic nucleus. NeuroReport, 11(5), 907-912.

    PLC β4-independent Ca2+ rise via muscarinic receptors in the mouse suprachiasmatic nucleus. / Ikeda, Masayuki; Sugiyama, Takashi; Suzuki, Kaoru; Moriya, Takahiro; Shibata, Shigenobu; Katsuki, Motoya; Allen, Charles N.; Yoshioka, Tohru.

    :: NeuroReport, 巻 11, 番号 5, 07.04.2000, p. 907-912.

    研究成果: Article

    Ikeda, M, Sugiyama, T, Suzuki, K, Moriya, T, Shibata, S, Katsuki, M, Allen, CN & Yoshioka, T 2000, 'PLC β4-independent Ca2+ rise via muscarinic receptors in the mouse suprachiasmatic nucleus', NeuroReport, 巻. 11, 番号 5, pp. 907-912.
    Ikeda M, Sugiyama T, Suzuki K, Moriya T, Shibata S, Katsuki M その他. PLC β4-independent Ca2+ rise via muscarinic receptors in the mouse suprachiasmatic nucleus. NeuroReport. 2000 4 7;11(5):907-912.
    Ikeda, Masayuki ; Sugiyama, Takashi ; Suzuki, Kaoru ; Moriya, Takahiro ; Shibata, Shigenobu ; Katsuki, Motoya ; Allen, Charles N. ; Yoshioka, Tohru. / PLC β4-independent Ca2+ rise via muscarinic receptors in the mouse suprachiasmatic nucleus. :: NeuroReport. 2000 ; 巻 11, 番号 5. pp. 907-912.
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    abstract = "Cholinergic regulation of the suprachiasmatic nucleus (SCN) has been extensively studied although the intracellular signaling mechanisms are not well understood. We examined immunostaining for phospholipase C-β (PLC-β) families that couple to muscarinic acetylcholine receptors (mAChR) and demonstrated the expression of PLC-βI and β4 in the mouse SCN. Ca2+ imaging analysis indicated that the MI-mAChR antagonist, pirenzepine blocked carbachol-induced Ca2+ elevation in the SCN and the response was equivalent between the wild type and the PLC-β4-knockout mice. In addition, the knockout mice displayed locomotor and temperature rhythms coupling to 24 h light/dark cycles. Therefore, it was proposed that PLC-βI but not PLC-β4 was involved in the mAChR-mediated Ca2+ signaling in the SCN. (C) 2000 Lippincott Williams and Wilkins.",
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    AU - Sugiyama, Takashi

    AU - Suzuki, Kaoru

    AU - Moriya, Takahiro

    AU - Shibata, Shigenobu

    AU - Katsuki, Motoya

    AU - Allen, Charles N.

    AU - Yoshioka, Tohru

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    N2 - Cholinergic regulation of the suprachiasmatic nucleus (SCN) has been extensively studied although the intracellular signaling mechanisms are not well understood. We examined immunostaining for phospholipase C-β (PLC-β) families that couple to muscarinic acetylcholine receptors (mAChR) and demonstrated the expression of PLC-βI and β4 in the mouse SCN. Ca2+ imaging analysis indicated that the MI-mAChR antagonist, pirenzepine blocked carbachol-induced Ca2+ elevation in the SCN and the response was equivalent between the wild type and the PLC-β4-knockout mice. In addition, the knockout mice displayed locomotor and temperature rhythms coupling to 24 h light/dark cycles. Therefore, it was proposed that PLC-βI but not PLC-β4 was involved in the mAChR-mediated Ca2+ signaling in the SCN. (C) 2000 Lippincott Williams and Wilkins.

    AB - Cholinergic regulation of the suprachiasmatic nucleus (SCN) has been extensively studied although the intracellular signaling mechanisms are not well understood. We examined immunostaining for phospholipase C-β (PLC-β) families that couple to muscarinic acetylcholine receptors (mAChR) and demonstrated the expression of PLC-βI and β4 in the mouse SCN. Ca2+ imaging analysis indicated that the MI-mAChR antagonist, pirenzepine blocked carbachol-induced Ca2+ elevation in the SCN and the response was equivalent between the wild type and the PLC-β4-knockout mice. In addition, the knockout mice displayed locomotor and temperature rhythms coupling to 24 h light/dark cycles. Therefore, it was proposed that PLC-βI but not PLC-β4 was involved in the mAChR-mediated Ca2+ signaling in the SCN. (C) 2000 Lippincott Williams and Wilkins.

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    KW - Locomotor activity

    KW - Muscarinic acetylcholine receptor

    KW - Phospholipase C-β

    KW - Pirenzepine

    KW - Suprachiasmatic nucleus

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