PLC β4-independent Ca2+ rise via muscarinic receptors in the mouse suprachiasmatic nucleus

Masayuki Ikeda*, Takashi Sugiyama, Kaoru Suzuki, Takahiro Moriya, Shigenobu Shibata, Motoya Katsuki, Charles N. Allen, Tohru Yoshioka

*この研究の対応する著者

研究成果: Article査読

14 被引用数 (Scopus)

抄録

Cholinergic regulation of the suprachiasmatic nucleus (SCN) has been extensively studied although the intracellular signaling mechanisms are not well understood. We examined immunostaining for phospholipase C-β (PLC-β) families that couple to muscarinic acetylcholine receptors (mAChR) and demonstrated the expression of PLC-βI and β4 in the mouse SCN. Ca2+ imaging analysis indicated that the MI-mAChR antagonist, pirenzepine blocked carbachol-induced Ca2+ elevation in the SCN and the response was equivalent between the wild type and the PLC-β4-knockout mice. In addition, the knockout mice displayed locomotor and temperature rhythms coupling to 24 h light/dark cycles. Therefore, it was proposed that PLC-βI but not PLC-β4 was involved in the mAChR-mediated Ca2+ signaling in the SCN. (C) 2000 Lippincott Williams and Wilkins.

本文言語English
ページ(範囲)907-912
ページ数6
ジャーナルNeuroReport
11
5
DOI
出版ステータスPublished - 2000 4月 7

ASJC Scopus subject areas

  • 神経科学(全般)

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