Polymicrobial Amniotic Fluid Infection with Mycoplasma/Ureaplasma and Other Bacteria Induces Severe Intra-Amniotic Inflammation Associated with Poor Perinatal Prognosis in Preterm Labor

Noriko Yoneda, Satoshi Yoneda, Hideki Niimi, Tomohiro Ueno, Shirou Hayashi, Mika Ito, Arihiro Shiozaki, Daichi Urushiyama, Kenichiro Hata, Wataru Suda, Masahira Hattori, Mika Kigawa, Isao Kitajima, Shigeru Saito*

*この研究の対応する著者

    研究成果: Article査読

    42 被引用数 (Scopus)

    抄録

    Problem: To study the relationship between perinatal prognosis in cases of preterm labor (PTL) and polymicrobial infection in amniotic fluid (AF) and intra-amniotic (IA) inflammation using a highly sensitive and reliable PCR-based method. Method of Study: To detect prokaryotes using a nested PCR-based method, eukaryote-made thermostable DNA polymerase without bacterial DNA contamination was used in combination with bacterial universal primers. We collected AF aseptically from 118 PTL cases and 50 term subjects. Results: The prevalence of microorganisms was 33% (39/118) by PCR and only 7.6% (9/118) by culture. PTL caused by a combination of positive Mycoplasma/Ureaplasma and other bacteria had significantly higher AF IL-8 levels and a significantly shorter amniocentesis-to-delivery interval. Conclusions: Our newly established PCR method is useful for detecting IA microorganisms. Polymicrobial infection with Mycoplasma/Ureaplasma and other bacteria induces severe IA inflammation associated with poor perinatal prognosis in PTL.

    本文言語English
    ページ(範囲)112-125
    ページ数14
    ジャーナルAmerican Journal of Reproductive Immunology
    75
    2
    DOI
    出版ステータスPublished - 2016 2月 1

    ASJC Scopus subject areas

    • 免疫学
    • 免疫アレルギー学
    • 産婦人科学
    • 生殖医学

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