Polymicrobial Amniotic Fluid Infection with Mycoplasma/Ureaplasma and Other Bacteria Induces Severe Intra-Amniotic Inflammation Associated with Poor Perinatal Prognosis in Preterm Labor

Noriko Yoneda, Satoshi Yoneda, Hideki Niimi, Tomohiro Ueno, Shirou Hayashi, Mika Ito, Arihiro Shiozaki, Daichi Urushiyama, Kenichiro Hata, Wataru Suda, Masahira Hattori, Mika Kigawa, Isao Kitajima, Shigeru Saito

    研究成果: Article

    29 引用 (Scopus)

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    Problem: To study the relationship between perinatal prognosis in cases of preterm labor (PTL) and polymicrobial infection in amniotic fluid (AF) and intra-amniotic (IA) inflammation using a highly sensitive and reliable PCR-based method. Method of Study: To detect prokaryotes using a nested PCR-based method, eukaryote-made thermostable DNA polymerase without bacterial DNA contamination was used in combination with bacterial universal primers. We collected AF aseptically from 118 PTL cases and 50 term subjects. Results: The prevalence of microorganisms was 33% (39/118) by PCR and only 7.6% (9/118) by culture. PTL caused by a combination of positive Mycoplasma/Ureaplasma and other bacteria had significantly higher AF IL-8 levels and a significantly shorter amniocentesis-to-delivery interval. Conclusions: Our newly established PCR method is useful for detecting IA microorganisms. Polymicrobial infection with Mycoplasma/Ureaplasma and other bacteria induces severe IA inflammation associated with poor perinatal prognosis in PTL.

    元の言語English
    ページ(範囲)112-125
    ページ数14
    ジャーナルAmerican Journal of Reproductive Immunology
    75
    発行部数2
    DOI
    出版物ステータスPublished - 2016 2 1

      フィンガープリント

    ASJC Scopus subject areas

    • Immunology
    • Immunology and Allergy
    • Obstetrics and Gynaecology
    • Reproductive Medicine

    これを引用

    Yoneda, N., Yoneda, S., Niimi, H., Ueno, T., Hayashi, S., Ito, M., Shiozaki, A., Urushiyama, D., Hata, K., Suda, W., Hattori, M., Kigawa, M., Kitajima, I., & Saito, S. (2016). Polymicrobial Amniotic Fluid Infection with Mycoplasma/Ureaplasma and Other Bacteria Induces Severe Intra-Amniotic Inflammation Associated with Poor Perinatal Prognosis in Preterm Labor. American Journal of Reproductive Immunology, 75(2), 112-125. https://doi.org/10.1111/aji.12456