Poly(N-isopropylacrylamide) Carrying Double-Stranded DNA for Affinity Separation of Genotoxins

Daisuke Umeno, Mizuo Maeda

研究成果: Article査読

25 被引用数 (Scopus)

抄録

We immobilized double-stranded DNA on poly(N-isopropylacrylamide), which is temperature-sensitive and gives precipitates from an aqueous solution when heated over 31°C. Photochemical conjugation with a vinyl derivative of psoralen was demonstrated to make the DNA polymerizable with a vinyl monomer. The radical copolymerization with N-isopropylacrylamide gave the temperature-responsive conjugate. The conjugate was shown to capture ethidium, a DNA-binding genotoxin, and precipitate with it when heated. About 95% of ethidium was separated from its highly diluted solution (3 ppm).

本文言語English
ページ(範囲)553-556
ページ数4
ジャーナルAnalytical Sciences
13
4
DOI
出版ステータスPublished - 1997
外部発表はい

ASJC Scopus subject areas

  • Analytical Chemistry

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