Position-specific release of DNA from a chip by using photothermal denaturation

Kazunori Okano*, Kenji Yasuda, Shin'ichi Ishiwata

*この研究の対応する著者

研究成果査読

8 被引用数 (Scopus)

抄録

A photothermal method to recover specific DNA fragments fixed in place on a DNA chip is described. This method uses infrared (IR) laser irradiation to thermally denature and release specific DNA immobilized in a specific area of a chip. A 1053-nm IR laser beam with an intensity of 10-100 mW is focused on the target area at a resolution of 10 μm, and the DNA fragments are released from the chip surface. We have demonstrated that DNA fragments containing different numbers of base pairs (231-799 bp) fixed in place on the DNA chip can be separately recovered. There are enough quantities of recovered DNA fragments that can be amplified by using polymerase chain reaction (PCR). The photothermal method coupled with the DNA chip can therefore be used in highly sensitive purification of DNA and will have many applications in the DNA chip technology.

本文言語English
ページ(範囲)88-94
ページ数7
ジャーナルSensors and Actuators, B: Chemical
64
1-3
DOI
出版ステータスPublished - 2000 6 10
外部発表はい
イベントTransducers '99 - 10th International Conference on Solid-State Sensors and Actuators - Sendai, Japan
継続期間: 1999 6 71999 6 10

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 器械工学
  • 凝縮系物理学
  • 表面、皮膜および薄膜
  • 金属および合金
  • 電子工学および電気工学
  • 材料化学

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