TY - JOUR
T1 - Position-specific release of DNA from a chip by using photothermal denaturation
AU - Okano, Kazunori
AU - Yasuda, Kenji
AU - Ishiwata, Shin'ichi
N1 - Funding Information:
The authors thank Professor Sakaki for kindly supplying the human genome DNA. This research was partially supported by Grants-in-Aid for Scientific Research, for Scientific Research on Priority Areas and for the High-Tech Research Center Project from the Ministry of Education, Science, Sports and Culture of Japan.
PY - 2000/6/10
Y1 - 2000/6/10
N2 - A photothermal method to recover specific DNA fragments fixed in place on a DNA chip is described. This method uses infrared (IR) laser irradiation to thermally denature and release specific DNA immobilized in a specific area of a chip. A 1053-nm IR laser beam with an intensity of 10-100 mW is focused on the target area at a resolution of 10 μm, and the DNA fragments are released from the chip surface. We have demonstrated that DNA fragments containing different numbers of base pairs (231-799 bp) fixed in place on the DNA chip can be separately recovered. There are enough quantities of recovered DNA fragments that can be amplified by using polymerase chain reaction (PCR). The photothermal method coupled with the DNA chip can therefore be used in highly sensitive purification of DNA and will have many applications in the DNA chip technology.
AB - A photothermal method to recover specific DNA fragments fixed in place on a DNA chip is described. This method uses infrared (IR) laser irradiation to thermally denature and release specific DNA immobilized in a specific area of a chip. A 1053-nm IR laser beam with an intensity of 10-100 mW is focused on the target area at a resolution of 10 μm, and the DNA fragments are released from the chip surface. We have demonstrated that DNA fragments containing different numbers of base pairs (231-799 bp) fixed in place on the DNA chip can be separately recovered. There are enough quantities of recovered DNA fragments that can be amplified by using polymerase chain reaction (PCR). The photothermal method coupled with the DNA chip can therefore be used in highly sensitive purification of DNA and will have many applications in the DNA chip technology.
KW - DNA chip
KW - Infrared laser
KW - PCR
KW - Photothermal denaturation
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U2 - 10.1016/S0925-4005(99)00489-X
DO - 10.1016/S0925-4005(99)00489-X
M3 - Conference article
AN - SCOPUS:0002040588
VL - 64
SP - 88
EP - 94
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
SN - 0925-4005
IS - 1-3
T2 - Transducers '99 - 10th International Conference on Solid-State Sensors and Actuators
Y2 - 7 June 1999 through 10 June 1999
ER -