Positive role of the mammalian TBPIP/HOP2 protein in DMC1-mediated homologous pairing

Rima Enomoto, Takashi Kinebuchi, Makoto Sato, Hideshi Yagi, Takehiko Shibata, Hitoshi Kurumizaka*, Shigeyuki Yokoyama

*この研究の対応する著者

    研究成果: Article査読

    32 被引用数 (Scopus)

    抄録

    In meiosis, homologous recombination preferentially occurs between homologous chromosomes rather than between sister chromatids, which is opposite to the bias of mitotic recombinational repair. The TBPIP/HOP2 protein is a factor that ensures the proper pairing of homologous chromosomes during meiosis. In the present study, we found that the purified mouse TBPIP/HOP2 protein stimulated homologous pairing catalyzed by the meiotic DMC1 recombinase in vitro. In contrast, TBPIP/HOP2 did not stimulate homologous pairing by RAD51, which is another homologous pairing protein acting in both meiotic and mitotic recombination. The positive effect of TBPIP/HOP2 in the DMC1-mediated homologous pairing was only observed when TBPIP/HOP2 first binds to double-stranded DNA, not to single-stranded DNA, before the initiation of the homologous pairing reaction. Deletion analyses revealed that the C-terminal basic region of TBPIP/HOP2 is required for efficient DNA binding and is also essential for its homologous pairing stimulation activity. Therefore, these results suggest that TBPIP/HOP2 directly binds to DNA and functions as an activator for DMC1 during the homologous pairing step in meiosis.

    本文言語English
    ページ(範囲)35263-35272
    ページ数10
    ジャーナルJournal of Biological Chemistry
    279
    34
    DOI
    出版ステータスPublished - 2004 8月 20

    ASJC Scopus subject areas

    • 生化学

    フィンガープリント

    「Positive role of the mammalian TBPIP/HOP2 protein in DMC1-mediated homologous pairing」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

    引用スタイル