Postmetamorphic changes in parvalbumin expression in the hindlimb skeletal muscle of the bullfrog, Rana catesbeiana

Takashi Hasebe, Kuniaki Umezawa, Minoru Sugita, Takeo Iwata, Kazutoshi Yamamoto, Takashi Obinata, Sakae Kikuyama*

*この研究の対応する著者

    研究成果: Article査読

    1 被引用数 (Scopus)

    抄録

    Anuran amphibians, animals that spend a terrestrial life after metamorphosis, exhibit a marked development of hindlimbs during and after metamorphosis. In order to see whether changes occur in the muscle protein components in the course of postmetamorphic development, we subjected gastrocnemius muscle extracts from growing froglets to two-dimensional electrophoresis (2DE). As a result, we found two proteins to undergo a change in level. One spot, indicating a molecular mass of approximately 12 kDa and an isoelectric point (pI) of 5.0 first became detectable at 45 days after metamorphosis. Another spot, corresponding to a protein of 11 kDa and pI 4.8, was prominent until the former spot appeared. N-terminal amino acid sequence analysis and comparison of the spots with those of parvalbumin (PA) revealed that these two proteins were PAα and PAβ. Northern blot analysis using PAα and PAβ cDNAs as probes revealed that the PAβ mRNA level declined whereas that of PAα mRNA rose as the frogs grew.

    本文言語English
    ページ(範囲)42-48
    ページ数7
    ジャーナルBiochimica et Biophysica Acta - Proteins and Proteomics
    1646
    1-2
    DOI
    出版ステータスPublished - 2003 3 21

    ASJC Scopus subject areas

    • 生化学
    • 生物理学
    • 遺伝学

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