Salivary cortisol concentration and its circadian variation were detected by optimizing the ionic concentration of a solution during the immobilization of aptamers on a field-effect transistor biosensor. This was achieved by modifying our previously developed technique of detecting uncharged cortisol using the transformation of negatively charged aptamers by controlling the aptamer spacing. This spacing control was achieved by synthesizing pseudo-cortisol-binding aptamers with a guaninequadruplex during the immobilization of the aptamers in a highly concentrated solution of K+.
ASJC Scopus subject areas
- 化学 (全般)