抄録
Quantification of small, dense low-density lipoprotein (sdLDL) cholesterol is clinically significant. We propose a practical technique to estimate the amount of sdLDL cholesterol using dynamic light scattering (DLS). An analytical solution in a closed form has newly been obtained to estimate the weight fraction of one species of scatterers in the DLS measurement of two species of scatterers. Using this solution, we can quantify the sdLDL cholesterol amount from the amounts of the low-density lipoprotein cholesterol and the high-density lipoprotein (HDL) cholesterol, which are commonly obtained through clinical tests. The accuracy of the proposed technique was confirmed experimentally using latex spheres with known size distributions. The applicability of the proposed technique was examined using samples of human blood serum. The possibility of estimating the sdLDL amount using the HDL data was demonstrated. These results suggest that the quantitative estimation of sdLDL amounts using DLS is feasible for point-of-care testing in clinical practice.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 265-272 |
| ページ数 | 8 |
| ジャーナル | Optical Review |
| 巻 | 23 |
| 発行部数 | 2 |
| DOI | |
| 出版物ステータス | Published - 2016 4 1 |
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ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
これを引用
Practical technique to quantify small, dense low-density lipoprotein cholesterol using dynamic light scattering. / Trirongjitmoah, Suchin; Iinaga, Kazuya; Sakurai, Toshihiro; Chiba, Hitoshi; Sriyudthsak, Mana; Shimizu, Koichi.
：: Optical Review, 巻 23, 番号 2, 01.04.2016, p. 265-272.研究成果: Article
}
TY - JOUR
T1 - Practical technique to quantify small, dense low-density lipoprotein cholesterol using dynamic light scattering
AU - Trirongjitmoah, Suchin
AU - Iinaga, Kazuya
AU - Sakurai, Toshihiro
AU - Chiba, Hitoshi
AU - Sriyudthsak, Mana
AU - Shimizu, Koichi
PY - 2016/4/1
Y1 - 2016/4/1
N2 - Quantification of small, dense low-density lipoprotein (sdLDL) cholesterol is clinically significant. We propose a practical technique to estimate the amount of sdLDL cholesterol using dynamic light scattering (DLS). An analytical solution in a closed form has newly been obtained to estimate the weight fraction of one species of scatterers in the DLS measurement of two species of scatterers. Using this solution, we can quantify the sdLDL cholesterol amount from the amounts of the low-density lipoprotein cholesterol and the high-density lipoprotein (HDL) cholesterol, which are commonly obtained through clinical tests. The accuracy of the proposed technique was confirmed experimentally using latex spheres with known size distributions. The applicability of the proposed technique was examined using samples of human blood serum. The possibility of estimating the sdLDL amount using the HDL data was demonstrated. These results suggest that the quantitative estimation of sdLDL amounts using DLS is feasible for point-of-care testing in clinical practice.
AB - Quantification of small, dense low-density lipoprotein (sdLDL) cholesterol is clinically significant. We propose a practical technique to estimate the amount of sdLDL cholesterol using dynamic light scattering (DLS). An analytical solution in a closed form has newly been obtained to estimate the weight fraction of one species of scatterers in the DLS measurement of two species of scatterers. Using this solution, we can quantify the sdLDL cholesterol amount from the amounts of the low-density lipoprotein cholesterol and the high-density lipoprotein (HDL) cholesterol, which are commonly obtained through clinical tests. The accuracy of the proposed technique was confirmed experimentally using latex spheres with known size distributions. The applicability of the proposed technique was examined using samples of human blood serum. The possibility of estimating the sdLDL amount using the HDL data was demonstrated. These results suggest that the quantitative estimation of sdLDL amounts using DLS is feasible for point-of-care testing in clinical practice.
KW - Dynamic light scattering
KW - Fraction
KW - Quantification
KW - Small, dense low-density lipoprotein
UR - http://www.scopus.com/inward/record.url?scp=84957546358&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84957546358&partnerID=8YFLogxK
U2 - 10.1007/s10043-016-0187-9
DO - 10.1007/s10043-016-0187-9
M3 - Article
AN - SCOPUS:84957546358
VL - 23
SP - 265
EP - 272
JO - Optical Review
JF - Optical Review
SN - 1340-6000
IS - 2
ER -