Pre-emptive Quality Control Protects the ER from Protein Overload via the Proximity of ERAD Components and SRP

Hisae Kadowaki, Atsushi Nagai, Takeshi Maruyama, Yasunari Takami, Pasjan Satrimafitrah, Hironori Kato, Arata Honda, Tomohisa Hatta, Tohru Natsume, Takashi Sato, Hirofumi Kai, Hidenori Ichijo, Hideki Nishitoh*

*この研究の対応する著者

研究成果: Article査読

37 被引用数 (Scopus)

抄録

Cells possess ER quality control systems to adapt to ER stress and maintain their function. ER-stress-induced pre-emptive quality control (ER pQC) selectively degrades ER proteins via translocational attenuation during ER stress. However, the molecular mechanism underlying this process remains unclear. Here, we find that most newly synthesized endogenous transthyretin proteins are rerouted to the cytosol without cleavage of the signal peptide, resulting in proteasomal degradation in hepatocytes during ER stress. Derlin family proteins (Derlins), which are ER-associated degradation components, reroute specific ER proteins, but not ER chaperones, from the translocon to the proteasome through interactions with the signal recognition particle (SRP). Moreover, the cytosolic chaperone Bag6 and the AAA-ATPase p97 contribute to the degradation of ER pQC substrates. These findings demonstrate that Derlins-mediated substrate-specific rerouting and Bag6- and p97-mediated effective degradation contribute to the maintenance of ER homeostasis without the need for translocation.

本文言語English
ページ(範囲)944-956
ページ数13
ジャーナルCell Reports
13
5
DOI
出版ステータスPublished - 2015 11 3
外部発表はい

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)

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