Precise comparison of protoporphyrin IX fluorescence spectra with pathological results for brain tumor tissue identification

Takehiro Ando, Etsuko Kobayashi, Hongen Liao, Takashi Maruyama, Yoshihiro Muragaki, Hiroshi Iseki, Osami Kubo, Ichiro Sakuma

研究成果: Article

36 引用 (Scopus)

抄録

Photodynamic diagnosis is used during glioma surgery. Although some studies have shown that the spectrum of fluorescence was efficient for precise tumor diagnosis, previous methods to characterize the spectrum have been problematic, which can lead to misdiagnosis. In this paper, we introduce a comparison technique to characterize spectrum from pathology and results of preliminary measurement using human brain tissues. We developed a spectrum scanning system that enables spectra measurement of raw tissues. Because tissue preparations retain the shape of the device holder, spectra can be compared precisely with pathological examination. As a preliminary analysis, we measured 13 sample tissues from five patients with brain tumors. The technique enabled us to measure spectra and compare them with pathological results. Some tissues exhibited a good relationship between spectra and pathological results. Although there were some false positive and false negative cases, false positive tissue had different spectra in which intensity of short-wavelength side was also high. The proposed technique provides an accurate comparison of quantitative fluorescence spectra with pathological results. We found that spectrum analysis may reduce false positive errors. These results will increase the accuracy of tumor tissue identification.

元の言語English
ページ(範囲)43-51
ページ数9
ジャーナルBrain Tumor Pathology
28
発行部数1
DOI
出版物ステータスPublished - 2011 2
外部発表Yes

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Brain Neoplasms
Fluorescence
protoporphyrin IX
Diagnostic Errors
Glioma
Neoplasms
Spectrum Analysis
Pathology
Equipment and Supplies
Brain

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Clinical Neurology

これを引用

Precise comparison of protoporphyrin IX fluorescence spectra with pathological results for brain tumor tissue identification. / Ando, Takehiro; Kobayashi, Etsuko; Liao, Hongen; Maruyama, Takashi; Muragaki, Yoshihiro; Iseki, Hiroshi; Kubo, Osami; Sakuma, Ichiro.

:: Brain Tumor Pathology, 巻 28, 番号 1, 02.2011, p. 43-51.

研究成果: Article

Ando, T, Kobayashi, E, Liao, H, Maruyama, T, Muragaki, Y, Iseki, H, Kubo, O & Sakuma, I 2011, 'Precise comparison of protoporphyrin IX fluorescence spectra with pathological results for brain tumor tissue identification', Brain Tumor Pathology, 巻. 28, 番号 1, pp. 43-51. https://doi.org/10.1007/s10014-010-0002-4
Ando, Takehiro ; Kobayashi, Etsuko ; Liao, Hongen ; Maruyama, Takashi ; Muragaki, Yoshihiro ; Iseki, Hiroshi ; Kubo, Osami ; Sakuma, Ichiro. / Precise comparison of protoporphyrin IX fluorescence spectra with pathological results for brain tumor tissue identification. :: Brain Tumor Pathology. 2011 ; 巻 28, 番号 1. pp. 43-51.
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AU - Muragaki, Yoshihiro

AU - Iseki, Hiroshi

AU - Kubo, Osami

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AB - Photodynamic diagnosis is used during glioma surgery. Although some studies have shown that the spectrum of fluorescence was efficient for precise tumor diagnosis, previous methods to characterize the spectrum have been problematic, which can lead to misdiagnosis. In this paper, we introduce a comparison technique to characterize spectrum from pathology and results of preliminary measurement using human brain tissues. We developed a spectrum scanning system that enables spectra measurement of raw tissues. Because tissue preparations retain the shape of the device holder, spectra can be compared precisely with pathological examination. As a preliminary analysis, we measured 13 sample tissues from five patients with brain tumors. The technique enabled us to measure spectra and compare them with pathological results. Some tissues exhibited a good relationship between spectra and pathological results. Although there were some false positive and false negative cases, false positive tissue had different spectra in which intensity of short-wavelength side was also high. The proposed technique provides an accurate comparison of quantitative fluorescence spectra with pathological results. We found that spectrum analysis may reduce false positive errors. These results will increase the accuracy of tumor tissue identification.

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