Preferences for phosphorylation sites in the retinoblastoma protein of D-type cyclin-dependent kinases, Cdk4 and Cdk6, in vitro

Tohru Takaki, Kazuhiro Fukasawa, Ikuko Suzuki-Takahashi, Kentaro Semba, Masatoshi Kitagawa, Yoichi Taya, Hiroshi Hirai

研究成果: Article査読

37 被引用数 (Scopus)

抄録

D-type cyclin-dependent kinases (Cdk4 and Cdk6) regulate the G1 to S phase progression of the mammalian cell cycle. It has been suggested that Cdk4 and Cdk6 may have distinct functions in vivo, even though they are indistinguishable biochemically. Here we show that although these Cdks phosphorylate multiple residues in pRB, they do so with different residue selectivities in vitro; Thr821 and Thr826 are preferentially phosphorylated by Cdk6 and Cdk4, respectively. This raises the possibility different substrate specificities lead to their different roles in the regulation of cellular events. Furthermore, our results indicate the new concept that Cdk itself contributes to substrate recognition.

本文言語English
ページ(範囲)381-386
ページ数6
ジャーナルJournal of biochemistry
137
3
DOI
出版ステータスPublished - 2005 3
外部発表はい

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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