Preferential reduction of the α-2-6-sialylation from cell surface N-glycans of human diploid fibroblastic cells by in vitro aging

Tomomi Tadokoro, Kiyotaka Yamamoto, Iku Kuwahara, Hirosuke Fujisawa, Masahiko Ikekita, Akiyoshi Taniguchi, Takeshi Sato, Kiyoshi Furukawa

研究成果: Article

12 引用 (Scopus)

抄録

Human diploid fibroblastic cell line, TIG-3, has a finite life span of about 80 population doubling levels (PDL), and is used for in vitro aging studies. Young cells (PDL 23) grew to higher cell densities at a higher growth rate than aged cells (PDL 77). When the electrophoretic mobility of cells was determined, the negative surface charge of the aged cells decreased significantly when compared to that of young cells. Lectin blot analysis of membrane glycoproteins showed that the α-2-6-sialylation but not the α-2-3-sialylation of N-glycans decreases markedly in the aged cells when compared to the young cells. In support of this observation, the cDNA microarray assay and reverse transcriptionpolymerase chain reaction (RT-PCR) analysis showed that the gene expression of the α-2,6-sialyltransferase I (ST6Gal I), which transfers sialic acid to galactose residues of N-glycans, decreases in the aged cells. These results indicate that the concordant decrease of the α-2,6-sialylation of N-glycans with the ST6Gal I gene expression is induced in TIG-3 cells by in vitro aging.

元の言語English
ページ(範囲)443-452
ページ数10
ジャーナルGlycoconjugate Journal
23
発行部数7-8
DOI
出版物ステータスPublished - 2006 11
外部発表Yes

Fingerprint

Diploidy
Polysaccharides
Aging of materials
Gene expression
Sialyltransferases
Electrophoretic mobility
Membrane Glycoproteins
N-Acetylneuraminic Acid
Surface charge
Microarrays
Galactose
Lectins
Assays
Complementary DNA
Cells
Population
Gene Expression
In Vitro Techniques
Oligonucleotide Array Sequence Analysis
beta-D-galactoside alpha 2-6-sialyltransferase

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

これを引用

Preferential reduction of the α-2-6-sialylation from cell surface N-glycans of human diploid fibroblastic cells by in vitro aging. / Tadokoro, Tomomi; Yamamoto, Kiyotaka; Kuwahara, Iku; Fujisawa, Hirosuke; Ikekita, Masahiko; Taniguchi, Akiyoshi; Sato, Takeshi; Furukawa, Kiyoshi.

:: Glycoconjugate Journal, 巻 23, 番号 7-8, 11.2006, p. 443-452.

研究成果: Article

Tadokoro, Tomomi ; Yamamoto, Kiyotaka ; Kuwahara, Iku ; Fujisawa, Hirosuke ; Ikekita, Masahiko ; Taniguchi, Akiyoshi ; Sato, Takeshi ; Furukawa, Kiyoshi. / Preferential reduction of the α-2-6-sialylation from cell surface N-glycans of human diploid fibroblastic cells by in vitro aging. :: Glycoconjugate Journal. 2006 ; 巻 23, 番号 7-8. pp. 443-452.
@article{550d2f17a5934d7db123a1a24ed6bbd8,
title = "Preferential reduction of the α-2-6-sialylation from cell surface N-glycans of human diploid fibroblastic cells by in vitro aging",
abstract = "Human diploid fibroblastic cell line, TIG-3, has a finite life span of about 80 population doubling levels (PDL), and is used for in vitro aging studies. Young cells (PDL 23) grew to higher cell densities at a higher growth rate than aged cells (PDL 77). When the electrophoretic mobility of cells was determined, the negative surface charge of the aged cells decreased significantly when compared to that of young cells. Lectin blot analysis of membrane glycoproteins showed that the α-2-6-sialylation but not the α-2-3-sialylation of N-glycans decreases markedly in the aged cells when compared to the young cells. In support of this observation, the cDNA microarray assay and reverse transcriptionpolymerase chain reaction (RT-PCR) analysis showed that the gene expression of the α-2,6-sialyltransferase I (ST6Gal I), which transfers sialic acid to galactose residues of N-glycans, decreases in the aged cells. These results indicate that the concordant decrease of the α-2,6-sialylation of N-glycans with the ST6Gal I gene expression is induced in TIG-3 cells by in vitro aging.",
keywords = "In vitro aging, N-glycans, Sialylation, ST6Gal I, TIG-3 cells",
author = "Tomomi Tadokoro and Kiyotaka Yamamoto and Iku Kuwahara and Hirosuke Fujisawa and Masahiko Ikekita and Akiyoshi Taniguchi and Takeshi Sato and Kiyoshi Furukawa",
year = "2006",
month = "11",
doi = "10.1007/s10719-006-7152-y",
language = "English",
volume = "23",
pages = "443--452",
journal = "Glycoconjugate Journal",
issn = "0282-0080",
publisher = "Springer Netherlands",
number = "7-8",

}

TY - JOUR

T1 - Preferential reduction of the α-2-6-sialylation from cell surface N-glycans of human diploid fibroblastic cells by in vitro aging

AU - Tadokoro, Tomomi

AU - Yamamoto, Kiyotaka

AU - Kuwahara, Iku

AU - Fujisawa, Hirosuke

AU - Ikekita, Masahiko

AU - Taniguchi, Akiyoshi

AU - Sato, Takeshi

AU - Furukawa, Kiyoshi

PY - 2006/11

Y1 - 2006/11

N2 - Human diploid fibroblastic cell line, TIG-3, has a finite life span of about 80 population doubling levels (PDL), and is used for in vitro aging studies. Young cells (PDL 23) grew to higher cell densities at a higher growth rate than aged cells (PDL 77). When the electrophoretic mobility of cells was determined, the negative surface charge of the aged cells decreased significantly when compared to that of young cells. Lectin blot analysis of membrane glycoproteins showed that the α-2-6-sialylation but not the α-2-3-sialylation of N-glycans decreases markedly in the aged cells when compared to the young cells. In support of this observation, the cDNA microarray assay and reverse transcriptionpolymerase chain reaction (RT-PCR) analysis showed that the gene expression of the α-2,6-sialyltransferase I (ST6Gal I), which transfers sialic acid to galactose residues of N-glycans, decreases in the aged cells. These results indicate that the concordant decrease of the α-2,6-sialylation of N-glycans with the ST6Gal I gene expression is induced in TIG-3 cells by in vitro aging.

AB - Human diploid fibroblastic cell line, TIG-3, has a finite life span of about 80 population doubling levels (PDL), and is used for in vitro aging studies. Young cells (PDL 23) grew to higher cell densities at a higher growth rate than aged cells (PDL 77). When the electrophoretic mobility of cells was determined, the negative surface charge of the aged cells decreased significantly when compared to that of young cells. Lectin blot analysis of membrane glycoproteins showed that the α-2-6-sialylation but not the α-2-3-sialylation of N-glycans decreases markedly in the aged cells when compared to the young cells. In support of this observation, the cDNA microarray assay and reverse transcriptionpolymerase chain reaction (RT-PCR) analysis showed that the gene expression of the α-2,6-sialyltransferase I (ST6Gal I), which transfers sialic acid to galactose residues of N-glycans, decreases in the aged cells. These results indicate that the concordant decrease of the α-2,6-sialylation of N-glycans with the ST6Gal I gene expression is induced in TIG-3 cells by in vitro aging.

KW - In vitro aging

KW - N-glycans

KW - Sialylation

KW - ST6Gal I

KW - TIG-3 cells

UR - http://www.scopus.com/inward/record.url?scp=84863710488&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863710488&partnerID=8YFLogxK

U2 - 10.1007/s10719-006-7152-y

DO - 10.1007/s10719-006-7152-y

M3 - Article

C2 - 16897185

AN - SCOPUS:84863710488

VL - 23

SP - 443

EP - 452

JO - Glycoconjugate Journal

JF - Glycoconjugate Journal

SN - 0282-0080

IS - 7-8

ER -