Preliminary study on the receptor of gonad-stimulating substance (GSS) as a gonadotropin of starfish

Masatoshi Mita*, Kazutoshi Yamamoto, Michiyasu Yoshikuni, Kaoru Ohno, Yoshitaka Nagahama

*この研究の対応する著者

    研究成果: Article査読

    18 被引用数 (Scopus)

    抄録

    Previously, the gonad-stimulating substance (GSS) which acts as the gonadotropin was purified from the starfish (Asterina pectinifera) and subsequently, its structure was deciphered. In this study, artificial GSS was synthesized and its interaction with the receptors was examined further. According to competitive experiments using radioiodinated and radioinert GSS in various tissues of A. pectinifera, high specific bindings were observed in the ovarian follicle cells and testicular interstitial cells. Scatchard plot analysis also showed that Kd values were about 4 nM in follicle cells and about 7 nM in interstitial cells. The numbers of binding sites in follicle cells were estimated to be about 3 pmoles/mg protein and in interstitial cells to be about 1 pmoles/mg protein. These strongly suggest that GSS receptors are distributed to follicle cells in female and interstitial cells in male, respectively.

    本文言語English
    ページ(範囲)299-301
    ページ数3
    ジャーナルGeneral and Comparative Endocrinology
    153
    1-3
    DOI
    出版ステータスPublished - 2007 8月

    ASJC Scopus subject areas

    • 内分泌学

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