Preparation of Bacterial Magnetite Particles for DNA Carriers

Haruko Takeyama, Satoko Kudo, Koji Sode, Moriyuki Nakamura, Tadashi Matsunaga

研究成果: Article査読

3 被引用数 (Scopus)

抄録

We have investigated the application of bacterial magnetite particles as DNA carriers for microprojectile based gene transfer. The magnetic bacterium, Aquaspirillum sp. AMB-1, can grow aerobically to high cell densities, allowing high yields of bacterial magnetite particles to be sonicated. The optimum sonication conditions were determined and found to be dependent on culture age. Excessive sonication damaged the magnetosome membrane surrounding magnetite particles. This decreased dispersion resulted in a reduced surface area available for DNA adsorption. When compared with gold, tungsten and non membrane coated magnetite, membrane coated magnetite was a better material for DNA binding (5 μg DNA/mg magnetite). The greatest amount of DNA was bound following treatment of membrane coated magnetite with glutaraldehyde-triamine prior to incubation with DNA.

本文言語English
ページ(範囲)319-325
ページ数7
ジャーナルKOBUNSHI RONBUNSHU
48
5
DOI
出版ステータスPublished - 1991 5
外部発表はい

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Materials Science (miscellaneous)
  • Environmental Science(all)
  • Polymers and Plastics

フィンガープリント 「Preparation of Bacterial Magnetite Particles for DNA Carriers」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル