Preparation of dehydrated powder of hemoglobin vesicles

Lin Wang, Shinji Takeoka, Eishun Tsuchida*, Satoru Tokuyama, Takahiro Mashiko, Tadashi Satoh

*この研究の対応する著者

    研究成果: Article査読

    10 被引用数 (Scopus)

    抄録

    The carbonyl hemoglobin (CO‐Hb), which was used to prevent denaturation (metHb) during the preparation of samples, was encapsulated into lipid vesicles constituted from unsaturated phospholipid, cholesterol and unsaturated fatty acid. Unsaturated components were polymerized by γ‐irradiation to enhance the stability of bilayer membrane. An aqueous dispersion of resulting Hb vesicles was freeze‐dried in the presence of saccharides (50–200 mM) to obtain a dehydrated powder of Hb vesicles. Change in the vesicle size, the leakage of encapsulated Hb and the oxidation of Hb to metHb were not observed. Therefore, the long‐term storage of Hb vesicles can be realized as a dry powder.

    本文言語English
    ページ(範囲)17-21
    ページ数5
    ジャーナルPolymers for Advanced Technologies
    3
    1
    DOI
    出版ステータスPublished - 1992

    ASJC Scopus subject areas

    • ポリマーおよびプラスチック

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