Preparation of human immune effector T cells containing iron-oxide nanoparticles

Hironori Iida, Kosuke Takayanagi, Takuya Nakanishi, Akiko Kume, Kouji Muramatsu, Yoshio Kiyohara, Yasuto Akiyama, Tetsuya Osaka

研究成果: Article査読

14 被引用数 (Scopus)

抄録

Preparation of human immune T cells containing iron-oxide nanoparticles was carried out for the development of magnetically mediated immunotherapy. Peripheral blood lymphocytes (PBLs) after the incubation with magnetite nanoparticles were found to contain measurable ferric ions, which suggested the incorporation of magnetite nanoparticles. Transmission electron microscopic (TEM) study indicated that the incorporation of magnetite nanoparticles was mediated by endocytosis of PBLs. Furthermore, the effects of dosages and diameter of magnetite nanoparticles on the magnetite incorporation were investigated, and it was demonstrated that the increase in dosage promoted the incorporation of nanoparticles and the uptake into PBLs was more effective for magnetite nanoparticles, which formed smaller aggregations in medium. Finally, the demonstration of magnetite incorporation into enriched T cells and tumor antigen-specific cytotoxic T lymphocyte (CTL) line promises the achievement of magnetically mediated immunotherapy with tumor-specific CTLs containing magnetic nanoparticles.

本文言語English
ページ(範囲)1123-1128
ページ数6
ジャーナルBiotechnology and bioengineering
101
6
DOI
出版ステータスPublished - 2008 12 15

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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