抄録
MagA is an iron-translocating protein found in the membranes of magnetic bacterium. Luciferase-bacterial magnetic particle (BMP) complexes were prepared by artificially inserting MagA-luciferase fusion proteins into the membranes of BMPs from Magnetospirillum magneticum strain AMB-1. Fusion proteins were from recombinant Escherichia coli membranes. MagA-Luc fusion proteins were integrated by sonication in vitro. Successful integration of fusion proteins was confirmed by luciferase luminescence on BMPs. Maximum luminescence was obtained after sonication for 3 min with a solution containing 300 mM NaCl, and is 18 times higher compared with recombinant Luc-BMPs generated using previously reported gene fusion techniques.
本文言語 | English |
---|---|
ページ(範囲) | 614-618 |
ページ数 | 5 |
ジャーナル | Biotechnology and Bioengineering |
巻 | 77 |
号 | 6 |
DOI | |
出版ステータス | Published - 2002 3月 20 |
外部発表 | はい |
ASJC Scopus subject areas
- バイオテクノロジー
- バイオエンジニアリング
- 応用微生物学とバイオテクノロジー