Processing of multiple forms of preprosodefrin in the abdominal gland of the red-bellied newt Cynops pyrrhogaster: Regional and individual differences in preprosodefrin gene expression

Takeo Iwata, J. Michael Conlon, Tomoaki Nakada, Fumiyo Toyoda, Kazutoshi Yamamoto, Sakae Kikuyama

    研究成果: Article

    18 引用 (Scopus)

    抄録

    Peptides derived from the post-translational processing of preprosodefrin were isolated from an extract of the abdominal glands of male red-bellied newts Cynops pyrrhogaster obtained 5 months prior to the onset of the breeding season. Structural characterization of the peptides showed that the pheromone sodefrin (SIPSKDALLK) is stored in a biologically inactive COOH-terminally extended form (SIPSKDALLKISA). It follows, therefore, that the activation of a protease that cleaves at a Lys-Ile bond to generate the active pheromone must occur by the time of onset of reproductive behavior. Additional peptides (representing preprosodefrin-(146-175)-peptide and preprosodefrin-(159-173)-peptide), that are derived from the precursor by cleavage at monobasic and dibasic processing sites, were also purified from the extract. The isolation of paralogs of these peptides, including an inactive COOH-terminally extended form of [Asn 10]sodefrin, provides evidence for the expression of multiple genes encoding preprosodefrin. PCR products derived from total RNAs from the abdominal gland of individual newts collected from three different regions of Japan were analyzed. The data confirm the existence of multiple genes encoding sodefrin and its variants whose expression varied according to the individuals and the regions. However, genes encoding sodefrin were found to be expressed in all the specimens sampled.

    元の言語English
    ページ(範囲)1537-1543
    ページ数7
    ジャーナルPeptides
    25
    発行部数9
    DOI
    出版物ステータスPublished - 2004 9

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    sodefrin
    Salamandridae
    Gene expression
    Individuality
    Gene Expression
    Peptides
    Gene encoding
    Processing
    Pheromones
    Reproductive Behavior
    Genes
    Breeding
    Japan
    Peptide Hydrolases
    Chemical activation
    RNA
    Polymerase Chain Reaction

    ASJC Scopus subject areas

    • Biochemistry
    • Endocrinology
    • Physiology
    • Cellular and Molecular Neuroscience

    これを引用

    Processing of multiple forms of preprosodefrin in the abdominal gland of the red-bellied newt Cynops pyrrhogaster : Regional and individual differences in preprosodefrin gene expression. / Iwata, Takeo; Conlon, J. Michael; Nakada, Tomoaki; Toyoda, Fumiyo; Yamamoto, Kazutoshi; Kikuyama, Sakae.

    :: Peptides, 巻 25, 番号 9, 09.2004, p. 1537-1543.

    研究成果: Article

    Iwata, Takeo ; Conlon, J. Michael ; Nakada, Tomoaki ; Toyoda, Fumiyo ; Yamamoto, Kazutoshi ; Kikuyama, Sakae. / Processing of multiple forms of preprosodefrin in the abdominal gland of the red-bellied newt Cynops pyrrhogaster : Regional and individual differences in preprosodefrin gene expression. :: Peptides. 2004 ; 巻 25, 番号 9. pp. 1537-1543.
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    abstract = "Peptides derived from the post-translational processing of preprosodefrin were isolated from an extract of the abdominal glands of male red-bellied newts Cynops pyrrhogaster obtained 5 months prior to the onset of the breeding season. Structural characterization of the peptides showed that the pheromone sodefrin (SIPSKDALLK) is stored in a biologically inactive COOH-terminally extended form (SIPSKDALLKISA). It follows, therefore, that the activation of a protease that cleaves at a Lys-Ile bond to generate the active pheromone must occur by the time of onset of reproductive behavior. Additional peptides (representing preprosodefrin-(146-175)-peptide and preprosodefrin-(159-173)-peptide), that are derived from the precursor by cleavage at monobasic and dibasic processing sites, were also purified from the extract. The isolation of paralogs of these peptides, including an inactive COOH-terminally extended form of [Asn 10]sodefrin, provides evidence for the expression of multiple genes encoding preprosodefrin. PCR products derived from total RNAs from the abdominal gland of individual newts collected from three different regions of Japan were analyzed. The data confirm the existence of multiple genes encoding sodefrin and its variants whose expression varied according to the individuals and the regions. However, genes encoding sodefrin were found to be expressed in all the specimens sampled.",
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    AU - Conlon, J. Michael

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