Production of γ‐linolenic acid from the marine green alga Chlorella sp. NKG 042401

Yuki Miura, Koji Sode, Noriyuki Nakamura, Naoki Matsunaga, Tadashi Matsunaga

研究成果: Article査読

18 被引用数 (Scopus)

抄録

Abstract γ‐Linolenic acid (GLA) production using a high GLA producing marine green alga, Chlorella sp. NKG 042401, was studied. GLA was presented in the galactolipid fraction (37.9%/total fatty acids). The effects of growth conditions on GLA production were studied. Optimum salinity for GLA production was 5 g 1−1, at which salinity the highest cell concentration was achieved, resulting in a 1.6‐fold increase in GLA productivity. Total fatty acid, however, was not drastically affected by change of salinity. Nitrogen starvation decreased the ratio of unsaturated fatty acids, and consequently GLA ratio in total fatty acid decreased. The urea adduct method was used to concentrate GLA from crude extract. As a result, after 5 sequential concentration procedures, GLA was concentrated 5‐fold with a yield of 49%.

本文言語English
ページ(範囲)163-167
ページ数5
ジャーナルFEMS Microbiology Letters
107
2-3
DOI
出版ステータスPublished - 1993 1 1
外部発表はい

ASJC Scopus subject areas

  • 微生物学
  • 分子生物学
  • 遺伝学

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