Production of antioxidant vitamins, β-carotene, vitamin C, and vitamin E, by two-step culture of Euglena gracilis Z

Haruko Takeyama, Akihisa Kanamaru, Yuko Yoshino, Hiroyuki Kakuta, Yoshiya Kawamura, Tadashi Matsunaga

研究成果: Article査読

67 被引用数 (Scopus)

抄録

Euglena gracilis Z is one of the few microorganisms which simultaneously produces antioxidant vitamins such as β-carotene and vitamins C and E. Photoheterotrophically cultured E. gracilis Z produced larger levels of biomass but with a lower content of antioxidant vitamins than photoautotrophically grown cultures. For efficient production of these vitamins, a two-step culture was performed. Cells were grown photoheterotrophically and then transferred to photoautotrophic conditions. When E. gracilis Z cells were grown in fed-batch culture under photoheterotrophic conditions, their density reached 19 g/L after 145 h. Subsequent transfer of these cells to photoautotrophic conditions increased vitamin content, enhancing the total vitamin yields, which were 71.0 mg/L of β-carotene, 30.1 mg/L of vitamin E, and 86.5 mg/L of vitamin C.

本文言語English
ページ(範囲)185-190
ページ数6
ジャーナルBiotechnology and bioengineering
53
2
DOI
出版ステータスPublished - 1997 1 20
外部発表はい

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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