Production of eicosapentaenoic acid by a bacterium isolated from mackerel intestines

Masamichi Akimoto*, Tetsuya Ishii, Koji Yamagaki, Kazuhisa Ohtaguchi, Kozo Koide, Kazunaga Yazawa

*この研究の対応する著者

研究成果: Article査読

33 被引用数 (Scopus)

抄録

Optimization of culture conditions for the growth rate, 5,8,11,14,17-cis-Eicosapentaenoic acid (EPA) content and EPA productivity of a bacterium isolated from Pacific mackerel intestines was investigated by use of a culture medium containing 1.00 wt% peptone and 0.50 wt% yeast extract in an artificial sea water (ASW). Cultivation temperature affected the growth rate and cellular EPA content of the bacterium. The cellular EPA content at 8°C was as great as 16.8 mg/g of dry cells, which was more than two times greater than that at 25°C (7.3 mg/g of dry cells), although the growth rate showed a maximum at 25°C. Both the yield of bacterial cells and the cellular EPA content at 25°C reached maximum values when the pH of the culture medium was nearly 7.0 and when the concentration of ASW was 100% (v/v). Under optimum culture conditions [25°C pH 7.0 and 100% (v/v) ASW], the amount of EPA accumulated in the cellular lipids reached 45.6 mg/L of culture broth after 8 hr.

本文言語English
ページ(範囲)911-915
ページ数5
ジャーナルJournal of the American Oil Chemists' Society
67
12
DOI
出版ステータスPublished - 1990 12月
外部発表はい

ASJC Scopus subject areas

  • 化学(その他)
  • 食品科学
  • 有機化学
  • 化学工学(全般)

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