Production of Immunoabsorbent Nanoparticles by Displaying Single-Domain Protein A on Potato Virus X

Kerstin Uhde-Holzem, Michael McBurney, Brylee David B. Tiu, Rigoberto C. Advincula, Rainer Fischer, Ulrich Commandeur*, Nicole F. Steinmetz

*この研究の対応する著者

研究成果: Article査読

23 被引用数 (Scopus)

抄録

The combination of antibodies with nanoparticles provides wide-ranging applications in biosensing. While several covalent presentation strategies have been established, there is need for alternative, non-covalent methods to provide a routine for scalable nanomanufacturing. We report the multivalent presentation of the B domain of Staphylococcus aureus protein A (SpAB) on potato virus X (PVX) nanoparticles. Three different synthetic strategies were used to obtain chimeric PVXSpAB filaments. The protein A fragments displayed on the surface of all three PVX chimeras remained fully functional as an immunoabsorbent for antibody capture enabling biosensing. The new biomaterials presented could find applications as diagnostic tools for biomedical or environmental monitoring.

本文言語English
ページ(範囲)231-241
ページ数11
ジャーナルMacromolecular Bioscience
16
2
DOI
出版ステータスPublished - 2016 2月 1
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • バイオエンジニアリング
  • 生体材料
  • ポリマーおよびプラスチック
  • 材料化学

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