Production of inbred and hybrid transgenic mice carrying large (>200 kb) foreign DNA fragments by intracytoplasmic sperm injection

Tomoharu Osada; Atsushi Toyoda; Stefan Moisyadi; Hidenori Akutsu; Masahira Hattori; Yoshiyuio Sakaki; Ryuzo Yanagimachi

doi:10.1002/mrd.20319

Production of inbred and hybrid transgenic mice carrying large (>200 kb) foreign DNA fragments by intracytoplasmic sperm injection

Tomoharu Osada, Atsushi Toyoda, Stefan Moisyadi, Hidenori Akutsu, Masahira Hattori, Yoshiyuio Sakaki, Ryuzo Yanagimachi

研究成果: Article

27 引用（Scopus）

抜粋

We have developed a mouse transgenesis technique that facilitates the insertion of large (∼200 kilo base pairs) DNA fragments into host genomes of both inbred and hybrid mice. Six inbred and three hybrid transgenic mice carrying a single bacterial artificial chromosome (BAC) clone with genes located in the Down syndrome critical region of human chromosome 21 were produced using this technology.

元の言語	English
ページ（範囲）	329-335
ページ数	7
ジャーナル	Molecular Reproduction and Development
巻	72
発行部数	3
DOI	https://doi.org/10.1002/mrd.20319
出版物ステータス	Published - 2005 11
外部発表	Yes

フィンガープリント

ASJC Scopus subject areas

Genetics
Developmental Biology
Cell Biology

これを引用

Osada, T., Toyoda, A., Moisyadi, S., Akutsu, H., Hattori, M., Sakaki, Y., & Yanagimachi, R. (2005). Production of inbred and hybrid transgenic mice carrying large (>200 kb) foreign DNA fragments by intracytoplasmic sperm injection. Molecular Reproduction and Development, 72(3), 329-335. https://doi.org/10.1002/mrd.20319

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abstract = "We have developed a mouse transgenesis technique that facilitates the insertion of large (∼200 kilo base pairs) DNA fragments into host genomes of both inbred and hybrid mice. Six inbred and three hybrid transgenic mice carrying a single bacterial artificial chromosome (BAC) clone with genes located in the Down syndrome critical region of human chromosome 21 were produced using this technology.",

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AU - Osada, Tomoharu

AU - Toyoda, Atsushi

AU - Moisyadi, Stefan

AU - Akutsu, Hidenori

AU - Hattori, Masahira

AU - Sakaki, Yoshiyuio

AU - Yanagimachi, Ryuzo

PY - 2005/11

Y1 - 2005/11

N2 - We have developed a mouse transgenesis technique that facilitates the insertion of large (∼200 kilo base pairs) DNA fragments into host genomes of both inbred and hybrid mice. Six inbred and three hybrid transgenic mice carrying a single bacterial artificial chromosome (BAC) clone with genes located in the Down syndrome critical region of human chromosome 21 were produced using this technology.

AB - We have developed a mouse transgenesis technique that facilitates the insertion of large (∼200 kilo base pairs) DNA fragments into host genomes of both inbred and hybrid mice. Six inbred and three hybrid transgenic mice carrying a single bacterial artificial chromosome (BAC) clone with genes located in the Down syndrome critical region of human chromosome 21 were produced using this technology.

KW - Bacterial artificial chromosome (BAC)

KW - Down syndrome

KW - Inbred mice

KW - Intracytoplasmic sperm injection (ICSI)

KW - Transgenesis

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JO - Molecular Reproduction and Development

JF - Molecular Reproduction and Development

SN - 1040-452X

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文献にアクセス

10.1002/mrd.20319