Production of inbred and hybrid transgenic mice carrying large (>200 kb) foreign DNA fragments by intracytoplasmic sperm injection

Tomoharu Osada; Atsushi Toyoda; Stefan Moisyadi; Hidenori Akutsu; Masahira Hattori; Yoshiyuio Sakaki; Ryuzo Yanagimachi

doi:10.1002/mrd.20319

Production of inbred and hybrid transgenic mice carrying large (>200 kb) foreign DNA fragments by intracytoplasmic sperm injection

Tomoharu Osada^*, Atsushi Toyoda, Stefan Moisyadi, Hidenori Akutsu, Masahira Hattori, Yoshiyuio Sakaki, Ryuzo Yanagimachi

^*この研究の対応する著者

研究成果: Article › 査読

27 被引用数 (Scopus)

抄録

We have developed a mouse transgenesis technique that facilitates the insertion of large (∼200 kilo base pairs) DNA fragments into host genomes of both inbred and hybrid mice. Six inbred and three hybrid transgenic mice carrying a single bacterial artificial chromosome (BAC) clone with genes located in the Down syndrome critical region of human chromosome 21 were produced using this technology.

本文言語	English
ページ（範囲）	329-335
ページ数	7
ジャーナル	Molecular Reproduction and Development
巻	72
号	3
DOI	https://doi.org/10.1002/mrd.20319
出版ステータス	Published - 2005 11月
外部発表	はい

ASJC Scopus subject areas

遺伝学
発生生物学
細胞生物学

文献へのアクセス

10.1002/mrd.20319

他のファイルとリンク

フィンガープリント

「Production of inbred and hybrid transgenic mice carrying large (>200 kb) foreign DNA fragments by intracytoplasmic sperm injection」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル

Production of inbred and hybrid transgenic mice carrying large (>200 kb) foreign DNA fragments by intracytoplasmic sperm injection. / Osada, Tomoharu; Toyoda, Atsushi; Moisyadi, Stefan; Akutsu, Hidenori; Hattori, Masahira; Sakaki, Yoshiyuio; Yanagimachi, Ryuzo.

In: Molecular Reproduction and Development, Vol. 72, No. 3, 11.2005, p. 329-335.

研究成果: Article › 査読

@article{395e773169b747d9bec794f5ef8f6264,

title = "Production of inbred and hybrid transgenic mice carrying large (>200 kb) foreign DNA fragments by intracytoplasmic sperm injection",

abstract = "We have developed a mouse transgenesis technique that facilitates the insertion of large (∼200 kilo base pairs) DNA fragments into host genomes of both inbred and hybrid mice. Six inbred and three hybrid transgenic mice carrying a single bacterial artificial chromosome (BAC) clone with genes located in the Down syndrome critical region of human chromosome 21 were produced using this technology.",

keywords = "Bacterial artificial chromosome (BAC), Down syndrome, Inbred mice, Intracytoplasmic sperm injection (ICSI), Transgenesis",

author = "Tomoharu Osada and Atsushi Toyoda and Stefan Moisyadi and Hidenori Akutsu and Masahira Hattori and Yoshiyuio Sakaki and Ryuzo Yanagimachi",

year = "2005",

month = nov,

doi = "10.1002/mrd.20319",

language = "English",

volume = "72",

pages = "329--335",

journal = "Gamete Research",

issn = "1040-452X",

publisher = "Wiley-Liss Inc.",

number = "3",

}

TY - JOUR

T1 - Production of inbred and hybrid transgenic mice carrying large (>200 kb) foreign DNA fragments by intracytoplasmic sperm injection

AU - Osada, Tomoharu

AU - Toyoda, Atsushi

AU - Moisyadi, Stefan

AU - Akutsu, Hidenori

AU - Hattori, Masahira

AU - Sakaki, Yoshiyuio

AU - Yanagimachi, Ryuzo

PY - 2005/11

Y1 - 2005/11

N2 - We have developed a mouse transgenesis technique that facilitates the insertion of large (∼200 kilo base pairs) DNA fragments into host genomes of both inbred and hybrid mice. Six inbred and three hybrid transgenic mice carrying a single bacterial artificial chromosome (BAC) clone with genes located in the Down syndrome critical region of human chromosome 21 were produced using this technology.

AB - We have developed a mouse transgenesis technique that facilitates the insertion of large (∼200 kilo base pairs) DNA fragments into host genomes of both inbred and hybrid mice. Six inbred and three hybrid transgenic mice carrying a single bacterial artificial chromosome (BAC) clone with genes located in the Down syndrome critical region of human chromosome 21 were produced using this technology.

KW - Bacterial artificial chromosome (BAC)

KW - Down syndrome

KW - Inbred mice

KW - Intracytoplasmic sperm injection (ICSI)

KW - Transgenesis

UR - http://www.scopus.com/inward/record.url?scp=25444515938&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=25444515938&partnerID=8YFLogxK

U2 - 10.1002/mrd.20319

DO - 10.1002/mrd.20319

M3 - Article

C2 - 16047391

AN - SCOPUS:25444515938

VL - 72

SP - 329

EP - 335

JO - Gamete Research

JF - Gamete Research

SN - 1040-452X

IS - 3

ER -