Production of Isopropyl cis-6-Hexadecenoate by Regiospecific Desaturation of Isopropyl Palmitate by a Double Mutant of a Rhodococcus Strain

Kenzo Koike, Mikio Takaiwa, Katsutoshi Ara, Shigeo Inoue, Yoshiharu Kimura, Susumu Ito

研究成果: Article査読

5 被引用数 (Scopus)

抄録

Resting cells of a double mutant noted as KSM-MT66, derived from Rhodococcus sp. strain KSM-B-3 by UV irradiation, were found to cis-desaturate isopropyl hexadecanoate, yielding isopropyl cis-6-hexadecenoate. Addition of sodium glutamate (1.0%), MgSO4 (2 mM), and thiamine (2 mM) increased the productivity of the unsaturated product in phosphate buffer. Optimal temperature and pH for the reaction were around 26°C and 7, respectively. Under the optimized conditions, more than 50 g/l of isopropyl cis-6-hexadecenoate was produced after a 3-day incubation by resting cells of the mutant. Thus, cis-6-hexadecenoic acid, the main component of human sebaceous lipids, can be manufactured economically by the rhodococcal bioconversion.

本文言語English
ページ(範囲)399-404
ページ数6
ジャーナルBioscience, Biotechnology and Biochemistry
64
2
DOI
出版ステータスPublished - 2000 1 1
外部発表はい

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

フィンガープリント 「Production of Isopropyl cis-6-Hexadecenoate by Regiospecific Desaturation of Isopropyl Palmitate by a Double Mutant of a Rhodococcus Strain」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル