Production of multi-subunit complexes on liposome through an E. coli cell-free expression system.

Yutetsu Kuruma, Toshiharu Suzuki, Takuya Ueda

研究成果: Article査読

16 被引用数 (Scopus)

抄録

Recently cell-free translation systems became a laboratory research tool to obtain an objective protein. However, a general method for in vitro protein synthesis and multi-subunit complex formation on lipid membrane has not been established. Here, we describe the procedure for the production of subcomplexes of F(o)F(1)-ATP synthase using a reconstructed cell-free translation system. As for the membrane part F(o) (a(1)b(2)c((10-15))), cosynthesis of c-subunit and UncI proteins, which are integral membrane proteins, and further c (11)-ring formation in lipid bilayer are performed by use of a liposomes-containing cell-free system. Moreover, as for the cytoplasm part F(1) (alpha(3)beta(3)gammadeltaepsilon), subcomplex formations are successfully achieved by mixing the translation products.

本文言語English
ページ(範囲)161-171
ページ数11
ジャーナルMethods in molecular biology (Clifton, N.J.)
607
DOI
出版ステータスPublished - 2010
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学

フィンガープリント

「Production of multi-subunit complexes on liposome through an E. coli cell-free expression system.」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル