抄録
A reversible salicylic acid decarboxylase (Sdc), found in the yeast Trichosporon moniliiforme WU-0401, is applicable for the production of p-aminosalicylic acid (PAS) from m-aminophenol (m-AP). For the high-yield production of PAS, used as an antituberculous agent, we developed F195Y, a genetically engineered Sdc mutant. We succeeded in selectively producing PAS from m-AP through an enzymatic KolbeSchmitt reaction in aqueous solution by using recombinant Escherichia coli cells expressing the gene encoding F195Y. We found that 70mM PAS was produced at 30 °C in 15h with a conversion yield of 70% (mol/mol).
本文言語 | English |
---|---|
ページ(範囲) | 206-208 |
ページ数 | 3 |
ジャーナル | Chemistry Letters |
巻 | 40 |
号 | 2 |
DOI | |
出版ステータス | Published - 2011 2月 4 |
ASJC Scopus subject areas
- 化学 (全般)