Production of squalene by squalene synthases and their truncated mutants in Escherichia coli

Akinori Katabami, Ling Li, Miki Iwasaki, Maiko Furubayashi, Kyoichi Saito, Daisuke Umeno*

*この研究の対応する著者

研究成果: Article査読

41 被引用数 (Scopus)

抄録

Squalene is a precursor of thousands of bioactive triterpenoids and also has industrial value as a lubricant, health-promoting agent, and/or drop-in biofuel. To establish an efficient Escherichia coli-based system for squalene production, we tested two different squalene synthases and their mutants in combination with precursor pathways. By co-expressing a chimeric mevalonate pathway with human or Thermosynechococcus squalene synthase, E.coli accumulated squalene up to 230mg/L or 55mg/g-DCW in flask culture. We also determined that a significant truncation of squalene synthase at the C-terminus retains partial cellular activity. The squalene-producing strain described herein represents a convenient platform for gene discovery and the construction of the pathway toward natural and non-natural hopanoids/steroids.

本文言語English
ページ(範囲)165-171
ページ数7
ジャーナルJournal of Bioscience and Bioengineering
119
2
DOI
出版ステータスPublished - 2015 2 1
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー

フィンガープリント

「Production of squalene by squalene synthases and their truncated mutants in Escherichia coli」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル