Prolactin augments luteinizing hormone binding to rat Leydig cells in serum-free culture

Minoru Takase*, Kazuyoshi Tsutsui, Seiichiro Kawashima

*この研究の対応する著者

研究成果: Article査読

抄録

Effect of prolactin on the testicular luteinizing hormone binding was studied in a serum-free culture system. By the collagenase digestion of decapsulated testes taken out from 25-day-old rats, Leydig cells were isolated and cultured for 7 days in DME/F12 (1:1) medium supplemented with insulin, transferrin, epidermal growth factor, and gentamicin. The cultured cells exhibited the 3β-hydroxysteroid dehydrogenase activity. Hill plots constructed from the data of competition experiment showed that the dissociation constant (Kd) was 0.33 × 10-10M. The Kd value was approximately the same as the known value for the rat testicular homogenates. When the Leydig cells were cultured with ovine prolactin for the last 3 days of 7-day culture period, the binding of luteinizing hormone increased to 1.7-fold ofthat in the control group. From these results it is concluded that prolactin acts to up-regulate the binding of luteinizing hormone to rat testicular Leydig cells in serum-free culture

本文言語English
ページ(範囲)511-517
ページ数7
ジャーナルJournal of Biosciences
21
4
DOI
出版ステータスPublished - 1996 6月
外部発表はい

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)
  • 農業および生物科学(全般)

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