Prolonged Oxygen-Carrying Ability of Hemoglobin Vesicles by Coencapsulation of Catalase in Vivo

Yuji Teramura, Hideo Kanazawa, Hiromi Sakai, Shinji Takeoka, Eishun Tsuchida

研究成果: Article査読

32 被引用数 (Scopus)

抄録

Hemoglobin (Hb) vesicles (particle diameter, ca. 250 nm) have been developed as Hb-based oxygen carriers in which a purified Hb solution is encapsulated with a phospholipid bilayer membrane. The oxidation of Hb to nonfunctional ferric Hb (metHb) was caused by reactive oxygen species, especially hydrogen peroxide (H2O2), in vivo in addition to autoxidation. We focused on the enzymatic elimination of H2O 2 to suppress the metHb formation in the Hb vesicles. In this study, we coencapsulated catalase with Hb within vesicles and studied the rate of metHb formation in vivo. The Hb vesicles containing 5.6 x 104 unit mL-1 catalase decreased the rate of metHb formation by half in comparison with Hb vesicles without catalase. We succeeded in prolonging the oxygen-carrying ability of the Hb vesicle in vivo by the coencapsulation of catalase.

本文言語English
ページ(範囲)1171-1176
ページ数6
ジャーナルBioconjugate Chemistry
14
6
DOI
出版ステータスPublished - 2003 11 1

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

フィンガープリント 「Prolonged Oxygen-Carrying Ability of Hemoglobin Vesicles by Coencapsulation of Catalase in Vivo」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル