Protein disulfide isomerase in the acrosome of rat sperm

M. Nakamura, Y. Michikawa, T. Baba, T. Mizunaga, H. Horti, S. Okinaga, T. Kobayashi

研究成果: Chapter

抄録

An endoplasmic reticulum (ER) protein, protein disulfide isomerase (PDI), was found to locate in the acrosome of rat spermatids and sperm by the avidin-biotin-peroxidasecomplex (ABC) method. Immunoblot analysis of PDI during developmental stages showed that protein was detected in the testis between the ages of 5 and 50 days. When Northern blot analysis was carried out using human PDI cDNA as a probe, a 2.6-kb PDI mRNA was found in both the spermatocytes and spermatids of rat testis. The PDI mRNA level was similar in both types of cells. It would appear that PDI may stabilize three-dimensional structures of secretory proteins in the acrosome of rat spermatids during spermiogenesis.

元の言語English
ホスト出版物のタイトルMolecular Andrology
ページ201-208
ページ数8
5
エディション4
出版物ステータスPublished - 1993
外部発表Yes

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Protein Disulfide-Isomerases
Acrosome
Spermatozoa
Spermatids
Testis
Messenger RNA
Proteins
Spermatocytes
Avidin
Spermatogenesis
Biotin
Endoplasmic Reticulum
Northern Blotting
Complementary DNA

ASJC Scopus subject areas

  • Endocrinology
  • Nephrology

これを引用

Nakamura, M., Michikawa, Y., Baba, T., Mizunaga, T., Horti, H., Okinaga, S., & Kobayashi, T. (1993). Protein disulfide isomerase in the acrosome of rat sperm. : Molecular Andrology (4 版, 巻 5, pp. 201-208)

Protein disulfide isomerase in the acrosome of rat sperm. / Nakamura, M.; Michikawa, Y.; Baba, T.; Mizunaga, T.; Horti, H.; Okinaga, S.; Kobayashi, T.

Molecular Andrology. 巻 5 4. 編 1993. p. 201-208.

研究成果: Chapter

Nakamura, M, Michikawa, Y, Baba, T, Mizunaga, T, Horti, H, Okinaga, S & Kobayashi, T 1993, Protein disulfide isomerase in the acrosome of rat sperm. : Molecular Andrology. 4 Edn, 巻. 5, pp. 201-208.
Nakamura M, Michikawa Y, Baba T, Mizunaga T, Horti H, Okinaga S その他. Protein disulfide isomerase in the acrosome of rat sperm. : Molecular Andrology. 4 版 巻 5. 1993. p. 201-208
Nakamura, M. ; Michikawa, Y. ; Baba, T. ; Mizunaga, T. ; Horti, H. ; Okinaga, S. ; Kobayashi, T. / Protein disulfide isomerase in the acrosome of rat sperm. Molecular Andrology. 巻 5 4. 版 1993. pp. 201-208
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