抄録
Thrombopoietin (TPO) is the major regulator of the proliferation and differentiation of megakaryocyte precursors through interaction with its receptor encoded by the c-mpl protooncogene. We established the human TPO-dependent leukemia cell line, UT-7/TPO. In these cells, TPO activated protein kinase C (PKC) in a time dependent manner. Subsequently, the c-myc gene was transiently induced to a maximal level 60-90 minutes after TPO exposure. In addition, we found that stimulating UT-7/TPO cells with TPO rapidly induces the significant accumulation of inositol 1,4,5-trisphosphate (Ins-P3), leading to the mobilization of calcium from intracellular stores. Taken together, the activation of PKC and subsequent c-myc gene induction are involved in the TPO-induced cellular response(s), presumably through the activation of PLC.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 290-294 |
| ページ数 | 5 |
| ジャーナル | Biochemical and Biophysical Research Communications |
| 巻 | 231 |
| 発行部数 | 2 |
| DOI | |
| 出版物ステータス | Published - 1997 2 13 |
| 外部発表 | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
これを引用
Protein kinase C and c-myc gene activation pathways in thrombopoietin signal transduction. / Kunitama, Masae; Shimizu, Ritsuko; Yamada, Minami; Kato, Takashi; Miyazaki, Hiroshi; Okada, Koji; Miura, Yasusada; Komatsu, Norio.
：: Biochemical and Biophysical Research Communications, 巻 231, 番号 2, 13.02.1997, p. 290-294.研究成果: Article
}
TY - JOUR
T1 - Protein kinase C and c-myc gene activation pathways in thrombopoietin signal transduction
AU - Kunitama, Masae
AU - Shimizu, Ritsuko
AU - Yamada, Minami
AU - Kato, Takashi
AU - Miyazaki, Hiroshi
AU - Okada, Koji
AU - Miura, Yasusada
AU - Komatsu, Norio
PY - 1997/2/13
Y1 - 1997/2/13
N2 - Thrombopoietin (TPO) is the major regulator of the proliferation and differentiation of megakaryocyte precursors through interaction with its receptor encoded by the c-mpl protooncogene. We established the human TPO-dependent leukemia cell line, UT-7/TPO. In these cells, TPO activated protein kinase C (PKC) in a time dependent manner. Subsequently, the c-myc gene was transiently induced to a maximal level 60-90 minutes after TPO exposure. In addition, we found that stimulating UT-7/TPO cells with TPO rapidly induces the significant accumulation of inositol 1,4,5-trisphosphate (Ins-P3), leading to the mobilization of calcium from intracellular stores. Taken together, the activation of PKC and subsequent c-myc gene induction are involved in the TPO-induced cellular response(s), presumably through the activation of PLC.
AB - Thrombopoietin (TPO) is the major regulator of the proliferation and differentiation of megakaryocyte precursors through interaction with its receptor encoded by the c-mpl protooncogene. We established the human TPO-dependent leukemia cell line, UT-7/TPO. In these cells, TPO activated protein kinase C (PKC) in a time dependent manner. Subsequently, the c-myc gene was transiently induced to a maximal level 60-90 minutes after TPO exposure. In addition, we found that stimulating UT-7/TPO cells with TPO rapidly induces the significant accumulation of inositol 1,4,5-trisphosphate (Ins-P3), leading to the mobilization of calcium from intracellular stores. Taken together, the activation of PKC and subsequent c-myc gene induction are involved in the TPO-induced cellular response(s), presumably through the activation of PLC.
UR - http://www.scopus.com/inward/record.url?scp=0031566275&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031566275&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1996.5969
DO - 10.1006/bbrc.1996.5969
M3 - Article
C2 - 9070265
AN - SCOPUS:0031566275
VL - 231
SP - 290
EP - 294
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -