Protein kinase C and c-myc gene activation pathways in thrombopoietin signal transduction

Masae Kunitama, Ritsuko Shimizu, Minami Yamada, Takashi Kato, Hiroshi Miyazaki, Koji Okada, Yasusada Miura, Norio Komatsu

研究成果: Article

19 引用 (Scopus)

抄録

Thrombopoietin (TPO) is the major regulator of the proliferation and differentiation of megakaryocyte precursors through interaction with its receptor encoded by the c-mpl protooncogene. We established the human TPO-dependent leukemia cell line, UT-7/TPO. In these cells, TPO activated protein kinase C (PKC) in a time dependent manner. Subsequently, the c-myc gene was transiently induced to a maximal level 60-90 minutes after TPO exposure. In addition, we found that stimulating UT-7/TPO cells with TPO rapidly induces the significant accumulation of inositol 1,4,5-trisphosphate (Ins-P3), leading to the mobilization of calcium from intracellular stores. Taken together, the activation of PKC and subsequent c-myc gene induction are involved in the TPO-induced cellular response(s), presumably through the activation of PLC.

元の言語English
ページ(範囲)290-294
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
231
発行部数2
DOI
出版物ステータスPublished - 1997 2 13
外部発表Yes

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Thrombopoietin
Signal transduction
myc Genes
Protein Kinase C
Transcriptional Activation
Signal Transduction
Genes
Chemical activation
Inositol 1,4,5-Trisphosphate
Megakaryocytes
Programmable logic controllers
Leukemia
Cells
Calcium
Cell Line

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

これを引用

Protein kinase C and c-myc gene activation pathways in thrombopoietin signal transduction. / Kunitama, Masae; Shimizu, Ritsuko; Yamada, Minami; Kato, Takashi; Miyazaki, Hiroshi; Okada, Koji; Miura, Yasusada; Komatsu, Norio.

:: Biochemical and Biophysical Research Communications, 巻 231, 番号 2, 13.02.1997, p. 290-294.

研究成果: Article

Kunitama, M, Shimizu, R, Yamada, M, Kato, T, Miyazaki, H, Okada, K, Miura, Y & Komatsu, N 1997, 'Protein kinase C and c-myc gene activation pathways in thrombopoietin signal transduction', Biochemical and Biophysical Research Communications, 巻. 231, 番号 2, pp. 290-294. https://doi.org/10.1006/bbrc.1996.5969
Kunitama, Masae ; Shimizu, Ritsuko ; Yamada, Minami ; Kato, Takashi ; Miyazaki, Hiroshi ; Okada, Koji ; Miura, Yasusada ; Komatsu, Norio. / Protein kinase C and c-myc gene activation pathways in thrombopoietin signal transduction. :: Biochemical and Biophysical Research Communications. 1997 ; 巻 231, 番号 2. pp. 290-294.
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