TY - JOUR
T1 - Protein kinase C and c-myc gene activation pathways in thrombopoietin signal transduction
AU - Kunitama, Masae
AU - Shimizu, Ritsuko
AU - Yamada, Minami
AU - Kato, Takashi
AU - Miyazaki, Hiroshi
AU - Okada, Koji
AU - Miura, Yasusada
AU - Komatsu, Norio
N1 - Funding Information:
This work was in part supported by Grants-in-Aid for Cancer Research and Scienti®c Research from the Ministry of Education, Science and Culture of Japan and by a grant from the Yamanouchi Foundation for Research on Metabolic Disorders. We thank Tomoko Ando for her technical assistance and Motoko Yoshida for preparing the manuscript.
PY - 1997/2/13
Y1 - 1997/2/13
N2 - Thrombopoietin (TPO) is the major regulator of the proliferation and differentiation of megakaryocyte precursors through interaction with its receptor encoded by the c-mpl protooncogene. We established the human TPO-dependent leukemia cell line, UT-7/TPO. In these cells, TPO activated protein kinase C (PKC) in a time dependent manner. Subsequently, the c-myc gene was transiently induced to a maximal level 60-90 minutes after TPO exposure. In addition, we found that stimulating UT-7/TPO cells with TPO rapidly induces the significant accumulation of inositol 1,4,5-trisphosphate (Ins-P3), leading to the mobilization of calcium from intracellular stores. Taken together, the activation of PKC and subsequent c-myc gene induction are involved in the TPO-induced cellular response(s), presumably through the activation of PLC.
AB - Thrombopoietin (TPO) is the major regulator of the proliferation and differentiation of megakaryocyte precursors through interaction with its receptor encoded by the c-mpl protooncogene. We established the human TPO-dependent leukemia cell line, UT-7/TPO. In these cells, TPO activated protein kinase C (PKC) in a time dependent manner. Subsequently, the c-myc gene was transiently induced to a maximal level 60-90 minutes after TPO exposure. In addition, we found that stimulating UT-7/TPO cells with TPO rapidly induces the significant accumulation of inositol 1,4,5-trisphosphate (Ins-P3), leading to the mobilization of calcium from intracellular stores. Taken together, the activation of PKC and subsequent c-myc gene induction are involved in the TPO-induced cellular response(s), presumably through the activation of PLC.
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U2 - 10.1006/bbrc.1996.5969
DO - 10.1006/bbrc.1996.5969
M3 - Article
C2 - 9070265
AN - SCOPUS:0031566275
VL - 231
SP - 290
EP - 294
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -