Protein resolution in elution chromatography using novel cation-exchange polymer-brush-immobilized particles

Takato Harayama, Yusuke Okamura, Yuichi Shimoda, Daisuke Umeno, Kyoichi Saito*, Naoyuki Shinohara, Noboru Kubota

*この研究の対応する著者

研究成果: Article査読

3 被引用数 (Scopus)

抄録

This study investigates improvements in high-resolution elution chromatography of proteins loaded to a bed charged by way of cation-exchange polymer-brush-immobilized particles. A sulfonic acid group as a cation-exchange group was introduced into a polymer brush grafted onto a polyethylene particle with an average diameter of 35 μm. A bed charged with the resulting cation-exchange polymer-brush-immobilized particles, SS bed, has a sulfonic acid group density of 0.36 mmol/mL-bed. A mixture of α-chymotripsinogen A (chy), cytochrome c (cyt), and lysozyme (lys) was loaded onto the SS bed. The subsequent linear gradient elution performance of the proteins adsorbed by the SS bed with 20 mM phosphate buffer (pH 6.0) and 1 M NaCl is compared with those by commercially available cation-exchange-bead-packed beds. The resolutions of the SS bed of both the chy/cyt and cyt/lys pairs at a linear velocity of 600 cm/h exceeded 1.9 at a loading amount of 0.8 mg/mL-bed, which was not achieved using the beds charged with SOURCE® 30S, POROS® 50HS, or Fractogel® EMD SO3- (s). Even at a loading amount of 12 mg/mL-bed, the resolutions of the SS bed for the chy/cyt and cyt/lys pairs at a linear velocity of 300 cm/h were 1.4 and 2.7, respectively.

本文言語English
ページ(範囲)896-902
ページ数7
ジャーナルJOURNAL OF CHEMICAL ENGINEERING OF JAPAN
45
11
DOI
出版ステータスPublished - 2012
外部発表はい

ASJC Scopus subject areas

  • 化学 (全般)
  • 化学工学(全般)

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