Protein synthesis by pure translation systems

Yoshihiro Shimizu, Takashi Kanamori, Takuya Ueda*

*この研究の対応する著者

研究成果: Article査読

258 被引用数 (Scopus)

抄録

We have developed a partially recombinant, cell-free, protein-synthesis system reconstituted solely from those essential elements of the Escherichia coli translation system, termed protein synthesis using recombinant elements (PURE). It provides higher reaction controllability in comparison to crude cell-free protein-synthesis systems for translation studies and biotechnology applications. The PURE system stands out among translation methods in that it provides not only a simple and unique "reverse" purification method of separating the synthesized protein from reaction mixture, but also that the system can be tailor-made according to individual protein requirements. In this paper, two new approaches to obtaining active proteins are described: the use of molecular chaperones, and modification of the reaction conditions. Several possible applications of the PURE system are also discussed.

本文言語English
ページ(範囲)299-304
ページ数6
ジャーナルMethods
36
3
DOI
出版ステータスPublished - 2005 7
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 生化学、遺伝学、分子生物学(全般)

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