Proteome analysis of shell matrix proteins in the brachiopod Laqueus rubellus

Yukinobu Isowa, Isao Sarashina, Kenshiro Oshima, Keiji Kito, Masahira Hattori, Kazuyoshi Endo

研究成果: Article

13 引用 (Scopus)

抄録

Background: The calcitic brachipod shells contain proteins that play pivotal roles in shell formation and are important in understanding the evolution of biomineralization. Here, we performed a large-scale exploration of shell matrix proteins in the brachiopod Laqueus rubellus. Results: A total of 40 proteins from the shell were identified. Apart from five proteins, i.e., ICP-1, MSP130, a cysteine protease, a superoxide dismutase, and actin, all other proteins identified had no homologues in public databases. Among these unknown proteins, one shell matrix protein was identified with a domain architecture that includes a NAD(P) binding domain, an ABC-type transport system, a transmembrane region, and an aspartic acid rich region, which has not been detected in other biominerals. We also identified pectin lyase-like, trypsin inhibitor, and saposin B functional domains in the amino acid sequences of the shell matrix proteins. The repertoire of brachiopod shell matrix proteins also contains two basic amino acid-rich proteins and proteins that have a variety of repeat sequences. Conclusions: Our study suggests an independent origin and unique mechanisms for brachiopod shell formation.

元の言語English
記事番号21
ジャーナルProteome Science
13
発行部数1
DOI
出版物ステータスPublished - 2015 8 15
外部発表Yes

Fingerprint

Proteome
Proteins
Saposins
Biomineralization
Basic Amino Acids
Trypsin Inhibitors
Cysteine Proteases
Aspartic Acid
NAD
Superoxide Dismutase
Actins
Amino Acid Sequence
Databases
Amino Acids

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

これを引用

Proteome analysis of shell matrix proteins in the brachiopod Laqueus rubellus. / Isowa, Yukinobu; Sarashina, Isao; Oshima, Kenshiro; Kito, Keiji; Hattori, Masahira; Endo, Kazuyoshi.

:: Proteome Science, 巻 13, 番号 1, 21, 15.08.2015.

研究成果: Article

Isowa, Yukinobu ; Sarashina, Isao ; Oshima, Kenshiro ; Kito, Keiji ; Hattori, Masahira ; Endo, Kazuyoshi. / Proteome analysis of shell matrix proteins in the brachiopod Laqueus rubellus. :: Proteome Science. 2015 ; 巻 13, 番号 1.
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