Prototype of micro flow cell for multi-analyte sandwich immunoassay

N. Honda, Shuichi Shoji, M. Isomura, Y. Ashihara, K. Akahori, H. Sato

    研究成果: Conference contribution

    1 被引用数 (Scopus)

    抄録

    A micro flow cell for sandwich immunoassay using Teflon (Asahi Glass Co.: CYTOP™) check valves for sample and reagent inlets is designed and fabricated. The check valves are used not only to prevent reverse flow of injected solutions but also to realize sequential injection of the sample and reagent by simple pressure control. The valve structure is designed to control the width and thickness of the sample and reagent flows at relatively small flow velocity that is useful to reduce effective sample and reagent volumes. We examined a fluorescent sandwich immunoassay for human AFP (α-fetoprotein) as a preliminary experiment and faster immunoassay within 30 min compared to the conventional one was realized in the micro flow cell.

    本文言語English
    ホスト出版物のタイトル2001 International Microprocesses and Nanotechnology Conference, MNC 2001
    出版社Institute of Electrical and Electronics Engineers Inc.
    ページ24-25
    ページ数2
    ISBN(印刷版)4891140178, 9784891140175
    DOI
    出版ステータスPublished - 2001
    イベントInternational Microprocesses and Nanotechnology Conference, MNC 2001 - Shimane, Japan
    継続期間: 2001 10 312001 11 2

    Other

    OtherInternational Microprocesses and Nanotechnology Conference, MNC 2001
    国/地域Japan
    CityShimane
    Period01/10/3101/11/2

    ASJC Scopus subject areas

    • バイオテクノロジー
    • 流体および伝熱
    • 電子工学および電気工学
    • 電子材料、光学材料、および磁性材料
    • 器械工学

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