Protrusive growth from giant liposomes driven by actin polymerization

Hidetake Miyata*, Shuji Nishiyama, Ken Ichirou Akashi, Kazuhiko Kinosita

*この研究の対応する著者

研究成果: Article査読

79 被引用数 (Scopus)

抄録

Development of protrusions in the cell is indispensable in the process of cell motility. Membrane protrusion has long been suggested to occur as a result of actin polymerization immediately beneath the cell membrane at the leading edge, but elucidation of the mechanism is insufficient because of the complexity of the cell. To study the mechanism, we prepared giant liposomes containing monomeric actin (100 or 200 μM) and introduced KCl into individual liposomes by an electroporation technique. On the electroporation, the giant liposomes deformed. Most importantly, protrusive structure grew from the liposomes containing 200 μM actin at rates (ranging from 0.3 to 0.7 μm/s) similar to those obtained in the cell. The deformation occurred in a time range (30 ~ 100 s) similar to that of actin polymerization monitored in a cuvette (ca. 50 s). Concomitant with deformation, Brownian motion of micron-sized particles entrapped in the liposomes almost ceased. From these observations, we conclude that actin polymerization in the liposomes caused the protrusive formation.

本文言語English
ページ(範囲)2048-2053
ページ数6
ジャーナルProceedings of the National Academy of Sciences of the United States of America
96
5
DOI
出版ステータスPublished - 1999 3 2
外部発表はい

ASJC Scopus subject areas

  • 遺伝学
  • 一般

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